4wv3
From Proteopedia
(Difference between revisions)
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| - | ''' | + | ==Crystal structure of the anthranilate CoA ligase AuaEII in complex with anthranoyl-AMP== |
| + | <StructureSection load='4wv3' size='340' side='right' caption='[[4wv3]], [[Resolution|resolution]] 2.60Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4wv3]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WV3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WV3 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3UK:5-O-[(S)-[(2-AMINOBENZOYL)OXY](HYDROXY)PHOSPHORYL]ADENOSINE'>3UK</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wv3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wv3 OCA], [http://pdbe.org/4wv3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wv3 RCSB], [http://www.ebi.ac.uk/pdbsum/4wv3 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The incorporation of nonacetate starter units during type II polyketide biosynthesis helps diversify natural products. Currently, there are few enzymatic strategies for the incorporation of nonacetate starter units in type II polyketide synthase (PKS) pathways. Here we report the crystal structure of AuaEII, the anthranilate:CoA ligase responsible for the generation of anthraniloyl-CoA, which is used as a starter unit by a type II PKS in aurachin biosynthesis. We present structural and protein sequence comparisons to other aryl:CoA ligases. We also compare the AuaEII crystal structure to a model of a CoA ligase homologue, AuaE, which is present in the same gene cluster. AuaE is predicted to have the same fold as AuaEII, but instead of CoA ligation, AuaE catalyzes acyl transfer of anthranilate from anthraniloyl-CoA to the acyl carrier protein (ACP). Together, this work provides insight into the molecular basis for starter unit selection of anthranilate in type II PKS biosynthesis. | ||
| - | + | Structural Insights into Anthranilate Priming during Type II Polyketide Biosynthesis.,Jackson DR, Tu SS, Nguyen M, Barajas JF, Schaub AJ, Krug D, Pistorius D, Luo R, Muller R, Tsai SC ACS Chem Biol. 2015 Nov 3. PMID:26473393<ref>PMID:26473393</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 4wv3" style="background-color:#fffaf0;"></div> | |
| - | [[Category: | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Barajas, J F]] | ||
| + | [[Category: Jackson, D R]] | ||
| + | [[Category: Krug, D]] | ||
[[Category: Muller, R]] | [[Category: Muller, R]] | ||
[[Category: Nguyen, M]] | [[Category: Nguyen, M]] | ||
| - | [[Category: Barajas, J.F]] | ||
| - | [[Category: Krug, D]] | ||
[[Category: Pistorious, D]] | [[Category: Pistorious, D]] | ||
| - | [[Category: Tu, S | + | [[Category: Tsai, S C]] |
| - | [[Category: | + | [[Category: Tu, S S]] |
| - | [[Category: | + | [[Category: Anthranilate]] |
| + | [[Category: Aurachin]] | ||
| + | [[Category: Coa ligase]] | ||
| + | [[Category: Ligase-ligase inhibitor complex]] | ||
| + | [[Category: Natural product biosynthesis]] | ||
Revision as of 10:01, 2 December 2015
Crystal structure of the anthranilate CoA ligase AuaEII in complex with anthranoyl-AMP
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