2bl1
From Proteopedia
| Line 5: | Line 5: | ||
==Overview== | ==Overview== | ||
| - | Modification of the lipid A moiety of lipopolysaccharide by the addition, of the sugar 4-amino-4-deoxy-L-arabinose (L-Ara4N) is a strategy adopted, by pathogenic Gram-negative bacteria to evade cationic antimicrobial, peptides produced by the innate immune system. L-Ara4N biosynthesis is, therefore a potential anti-infective target, because inhibiting its, synthesis would render certain pathogens more sensitive to the immune, system. The bifunctional enzyme ArnA, which is required for L-Ara4N, biosynthesis, catalyzes the NAD(+)-dependent oxidative decarboxylation of, UDP-glucuronic acid to generate a UDP-4'-keto-pentose sugar and also, catalyzes transfer of a formyl group from N-10-formyltetrahydrofolate to, the 4'-amine of UDP-L-Ara4N. We now report the crystal structure of the, ... | + | Modification of the lipid A moiety of lipopolysaccharide by the addition, of the sugar 4-amino-4-deoxy-L-arabinose (L-Ara4N) is a strategy adopted, by pathogenic Gram-negative bacteria to evade cationic antimicrobial, peptides produced by the innate immune system. L-Ara4N biosynthesis is, therefore a potential anti-infective target, because inhibiting its, synthesis would render certain pathogens more sensitive to the immune, system. The bifunctional enzyme ArnA, which is required for L-Ara4N, biosynthesis, catalyzes the NAD(+)-dependent oxidative decarboxylation of, UDP-glucuronic acid to generate a UDP-4'-keto-pentose sugar and also, catalyzes transfer of a formyl group from N-10-formyltetrahydrofolate to, the 4'-amine of UDP-L-Ara4N. We now report the crystal structure of the, N-terminal formyltransferase domain in a complex with uridine, monophosphate and N-5-formyltetrahydrofolate. Using this structure, we, identify the active site of formyltransfer in ArnA, including the key, catalytic residues Asn(102), His(104), and Asp(140). Additionally, we have, shown that residues Ser(433) and Glu(434) of the decarboxylase domain are, required for the oxidative decarboxylation of UDP-GlcUA. An E434Q mutant, is inactive, suggesting that chemical rather than steric properties of, this residue are crucial in the decarboxylation reaction. Our data suggest, that the decarboxylase domain catalyzes both hydride abstraction, (oxidation) from the C-4' position and the subsequent decarboxylation. |
==About this Structure== | ==About this Structure== | ||
| - | 2BL1 is a | + | 2BL1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with AZI, SO4 and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BL1 OCA]. |
==Reference== | ==Reference== | ||
| Line 30: | Line 30: | ||
[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 15:01:45 2007'' |
Revision as of 12:56, 5 November 2007
|
CRYSTAL STRUCTURE OF A PUTATIVE PHOSPHINOTHRICIN ACETYLTRANSFERASE (PA4866) FROM PSEUDOMONAS AERUGINOSA PAC1
Overview
Modification of the lipid A moiety of lipopolysaccharide by the addition, of the sugar 4-amino-4-deoxy-L-arabinose (L-Ara4N) is a strategy adopted, by pathogenic Gram-negative bacteria to evade cationic antimicrobial, peptides produced by the innate immune system. L-Ara4N biosynthesis is, therefore a potential anti-infective target, because inhibiting its, synthesis would render certain pathogens more sensitive to the immune, system. The bifunctional enzyme ArnA, which is required for L-Ara4N, biosynthesis, catalyzes the NAD(+)-dependent oxidative decarboxylation of, UDP-glucuronic acid to generate a UDP-4'-keto-pentose sugar and also, catalyzes transfer of a formyl group from N-10-formyltetrahydrofolate to, the 4'-amine of UDP-L-Ara4N. We now report the crystal structure of the, N-terminal formyltransferase domain in a complex with uridine, monophosphate and N-5-formyltetrahydrofolate. Using this structure, we, identify the active site of formyltransfer in ArnA, including the key, catalytic residues Asn(102), His(104), and Asp(140). Additionally, we have, shown that residues Ser(433) and Glu(434) of the decarboxylase domain are, required for the oxidative decarboxylation of UDP-GlcUA. An E434Q mutant, is inactive, suggesting that chemical rather than steric properties of, this residue are crucial in the decarboxylation reaction. Our data suggest, that the decarboxylase domain catalyzes both hydride abstraction, (oxidation) from the C-4' position and the subsequent decarboxylation.
About this Structure
2BL1 is a Single protein structure of sequence from Pseudomonas aeruginosa with AZI, SO4 and GOL as ligands. Structure known Active Site: AC1. Full crystallographic information is available from OCA.
Reference
Structure and function of both domains of ArnA, a dual function decarboxylase and a formyltransferase, involved in 4-amino-4-deoxy-L-arabinose biosynthesis., Williams GJ, Breazeale SD, Raetz CR, Naismith JH, J Biol Chem. 2005 Jun 17;280(24):23000-8. Epub 2005 Apr 4. PMID:15809294
Page seeded by OCA on Mon Nov 5 15:01:45 2007
Categories: Pseudomonas aeruginosa | Single protein | Agha, R. | Brown, P.R. | Davies, A.M. | Sutton, B.J. | TATA, R. | AZI | GOL | SO4 | Gcn5 family | Gnat | Hypothetical protein | N-acetyltransferase | Phosphinothricin | Transferase
