1ajy
From Proteopedia
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|PDB= 1ajy |SIZE=350|CAPTION= <scene name='initialview01'>1ajy</scene> | |PDB= 1ajy |SIZE=350|CAPTION= <scene name='initialview01'>1ajy</scene> | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | + | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ajy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ajy OCA], [http://www.ebi.ac.uk/pdbsum/1ajy PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ajy RCSB]</span> | ||
}} | }} | ||
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[[Category: Wagner, G.]] | [[Category: Wagner, G.]] | ||
[[Category: Walters, K J.]] | [[Category: Walters, K J.]] | ||
| - | [[Category: ZN]] | ||
[[Category: put3]] | [[Category: put3]] | ||
[[Category: transcription regulation]] | [[Category: transcription regulation]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:41:54 2008'' |
Revision as of 15:41, 30 March 2008
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| Ligands: | |||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE AND MOBILITY OF THE PUT3 DIMER: A DNA PINCER, NMR, 13 STRUCTURES
Overview
The solution structure and backbone dynamics of the transcriptional activator PUT3 (31-100) has been characterized using NMR spectroscopy. PUT3 (31-100) contains three distinct domains: a cysteine zinc cluster, linker, and dimerization domain. The cysteine zinc cluster of PUT3 closely resembles the solution structure of GAL4, while the dimerization domain forms a long coiled-coil similar to that observed in the crystal structures of GAL4 and PPR1. However, the residues at the N-terminal end of the coiled-coil behave very differently in each of these proteins. A comparison of the structural elements within this region provides a model for the DNA binding specificity of these proteins. Furthermore, we have characterized the dynamics of PUT3 to find that the zinc cluster and dimerization domains have very diverse dynamics in solution. The dimerization domain behaves as a large protein, while the peripheral cysteine zinc clusters have dynamic properties similar to small proteins.
About this Structure
1AJY is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structure and mobility of the PUT3 dimer., Walters KJ, Dayie KT, Reece RJ, Ptashne M, Wagner G, Nat Struct Biol. 1997 Sep;4(9):744-50. PMID:9303003
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