3bt4
From Proteopedia
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<table><tr><td colspan='2'>[[3bt4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Antheraea_mylitta Antheraea mylitta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BT4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BT4 FirstGlance]. <br> | <table><tr><td colspan='2'>[[3bt4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Antheraea_mylitta Antheraea mylitta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BT4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BT4 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bt4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bt4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3bt4 RCSB], [http://www.ebi.ac.uk/pdbsum/3bt4 PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bt4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bt4 OCA], [http://pdbe.org/3bt4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3bt4 RCSB], [http://www.ebi.ac.uk/pdbsum/3bt4 PDBsum]</span></td></tr> |
</table> | </table> | ||
== Function == | == Function == | ||
[[http://www.uniprot.org/uniprot/FPI1_ANTMY FPI1_ANTMY]] Inhibits proteases from the fungi A.oryzae and R.oryzae, trypsin and chymotrypsin. Does not inhibit protease from the bacterium B.licheniformis or papain.<ref>PMID:14505696</ref> | [[http://www.uniprot.org/uniprot/FPI1_ANTMY FPI1_ANTMY]] Inhibits proteases from the fungi A.oryzae and R.oryzae, trypsin and chymotrypsin. Does not inhibit protease from the bacterium B.licheniformis or papain.<ref>PMID:14505696</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Indian tasar silk is produced by a wild insect called Antheraea mylitta. Insects do not have any antigen-antibody mediated immune system like vertebrates but they produce a wide variety of effector proteins and peptides possessing potent antifungal and antibacterial activity to combat microbial attack. Antheraea mylitta expresses a fungal protease inhibitor AmFPI-1, in the hemolymph that inhibits alkaline protease of Aspergillus oryzae for protection against fungal infection. AmFPI-1 is purified from the hemolymph, crystallized and the structure is solved using the single isomorphous replacement with anomalous scattering (SIRAS) method to a resolution of 2.1 A. AmFPI-1 is a single domain protein possessing a unique fold that consists of three helices and five beta strands stabilized by a network of six disulfide bonds. The reactive site of AmFPI-1 is located in the loop formed by residues 46-66, wherein Lys54 is the P(1) residue. Superimposition of the loop with reactive sites of other canonical protease inhibitors shows that reactive site conformation of AmFPI-1 is similar to them. The structure of AmFPI-1 provides a framework for the docking of a 1:1 complex between AmFPI-1 and alkaline protease. This study addresses the structural basis of AmFPI-1's specificity towards a fungal serine protease but not to mammalian trypsin and may help in designing specific inhibitors against fungal proteases. | ||
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| + | Crystal structure of a fungal protease inhibitor from Antheraea mylitta.,Roy S, Aravind P, Madhurantakam C, Ghosh AK, Sankaranarayanan R, Das AK J Struct Biol. 2009 Apr;166(1):79-87. PMID:19263521<ref>PMID:19263521</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 3bt4" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 07:22, 9 December 2015
Crystal Structure Analysis of AmFPI-1, fungal protease inhibitor from Antheraea mylitta
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