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2mmy
From Proteopedia
(Difference between revisions)
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2mmy]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MMY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MMY FirstGlance]. <br> | <table><tr><td colspan='2'>[[2mmy]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MMY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MMY FirstGlance]. <br> | ||
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mmy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mmy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2mmy RCSB], [http://www.ebi.ac.uk/pdbsum/2mmy PDBsum]</span></td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mmy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mmy OCA], [http://pdbe.org/2mmy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mmy RCSB], [http://www.ebi.ac.uk/pdbsum/2mmy PDBsum]</span></td></tr> |
</table> | </table> | ||
== Disease == | == Disease == | ||
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== Function == | == Function == | ||
[[http://www.uniprot.org/uniprot/RBP56_HUMAN RBP56_HUMAN]] RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II).<ref>PMID:19124016</ref> | [[http://www.uniprot.org/uniprot/RBP56_HUMAN RBP56_HUMAN]] RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II).<ref>PMID:19124016</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Human TATA binding protein associated factor 2 N (TAF15) and Fused in sarcoma (FUS) are nucleic acid binding proteins belonging to the conserved FET family of proteins. They are involved in diverse processes such as pre-mRNA splicing, mRNA transport, and DNA binding. The absence of information regarding the structural mechanism employed by the FET family in recognizing and discriminating their cognate and non-cognate RNA targets has hampered the attainment of consensus on modes of protein-RNA binding for this family. Our study provides a molecular basis of this RNA recognition using a combination of solution-state NMR spectroscopy, calorimetry, docking and molecular dynamics simulation. Analysis of TAF15-RRM solution structure and its binding with stem-loop RNA has yielded conclusive evidence of a non-canonical mode of RNA recognition. Rather than classical stacking interactions that occur across nitrogen bases and aromatic amino acids on ribonucleoprotein sites, moderate-affinity hydrogen bonding network between the nitrogen bases in the stem-loop RNA and a concave face on the RRM surface primarily mediate TAF15-RRM RNA interaction. We have compared the binding affinities across a set of single-stranded RNA oligonucleotides to conclusively establish that RNA binding is dependent upon structural elements in the RNA rather than sequence. | ||
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| + | Structural delineation of stem-loop RNA binding by human TAF15 protein.,Kashyap M, Ganguly AK, Bhavesh NS Sci Rep. 2015 Nov 27;5:17298. doi: 10.1038/srep17298. PMID:26612539<ref>PMID:26612539</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 2mmy" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: Bhavesh, N]] | + | [[Category: Bhavesh, N S]] |
[[Category: Ganguly, A K]] | [[Category: Ganguly, A K]] | ||
[[Category: Kashyap, M]] | [[Category: Kashyap, M]] | ||
Revision as of 07:28, 9 December 2015
Solution structure of the RNA recognition motif of human TAF15
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