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| | + | == Crystal structure of a novel two domain |
| | + | GH78 family a-rhamnosidase from Klebsiella |
| | + | oxytoca with rhamnose bound == |
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| | ==Your Heading Here (maybe something like 'Structure')== | | ==Your Heading Here (maybe something like 'Structure')== |
| - | <StructureSection load='4xhc' size='340' side='right' caption='Caption for this structure' scene=''> | + | <StructureSection load='4xhc' size='350' side='right' caption='X-ray crystal structure of the a-L-rhamnosidase from K. oxytoca (KoRha).' scene=''> |
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| - | Anything in this section will appear adjacent to the 3D structure and will be scrollable.
| + | a-L-rhamnosidases (E.C. 3.2.1.40) are found widely distributed |
| - | | + | in nature and have been reported in animals, |
| - | ' size='350' frame='true' align='right' caption='Insert caption here' scene='Insert optional scene name here' />==Your Heading Here (maybe something like 'Structure')==
| + | plants, yeast, fungi and bacteria, where they are responsible |
| - | <StructureSection load='3rec' size='350' side='right' caption='Escherichia coli reca protein-bound DNA (PDB entry [[3rec]])' scene=''>==Your Heading Here (maybe something like 'Structure')==
| + | for the cleavage of a-L-rhamnose from a wide range of |
| - | <StructureSection load='3rec' size='350' side='right' caption='Escherichia coli reca protein-bound DNA (PDB entry [[3rec]])' scene=''>
| + | compounds.1 a-L-rhamnose is found in plants and bacteria |
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| - | Anything in this section will appear adjacent to the 3D structure and will be scrollable.
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| - | </StructureSection>==Your Heading Here (maybe something like 'Structure')==
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| - | <StructureSection load='3rec' size='350' side='right' caption='Escherichia coli reca protein-bound DNA (PDB entry [[3rec]])' scene=''>==Your Heading Here (maybe something like 'Structure')==
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| - | <StructureSection load='3rec' size='350' side='right' caption='Escherichia coli reca protein-bound DNA (PDB entry [[3rec]])' scene=''>
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| - | Anything in this section will appear adjacent to the 3D structure and will be scrollable.
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| - | </StructureSection></StructureSection></StructureSection></StructureSection>== Crystal structure of a novel two domain GH78 family a-rhamnosidase from Klebsiella
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| - | oxytoca with rhamnose bound ==
| + | |
| - | <StructureSection load='1stp' size='340' side='right' caption='Caption for this structure' scene=''>
| + | |
| - | == Structure ==
| + | |
| - | The crystal structure of the GH78 family a-rhamnosidase from Klebsiella oxytoca (KoRha) has been determined at 2.7 A °
| + | |
| - | resolution with rhamnose bound in the active site of the catalytic domain. Curiously, the putative catalytic acid, Asp 222, is
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| - | preceded by an unusual non-proline cis-peptide bond which helps to project the carboxyl group into the active centre. This
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| - | KoRha homodimeric structure is significantly smaller than those of the other previously determined GH78 structures.
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| - | Nevertheless, the enzyme displays a-rhamnosidase activity when assayed in vitro, suggesting that the additional structural
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| - | domains found in the related enzymes are dispensible for function.
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| - | | + | |
| - | == Function ==
| + | |
| - | a-L-rhamnose is found in plants and bacteria | + | |
| | as components of polysaccharides, such as pectins,2 and | | as components of polysaccharides, such as pectins,2 and |
| | the O antigen polysaccharides, responsible for determining | | the O antigen polysaccharides, responsible for determining |
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| | and for the release of flavonoids from rhamnosylated precursors; | | and for the release of flavonoids from rhamnosylated precursors; |
| | in wine production they play a role in the hydrolysis | | in wine production they play a role in the hydrolysis |
| - | of glycosylated terpene aroma compounds.5 In the | + | of glycosylated terpene aroma compounds. |
| - | context of expanding and diversifying the suite of enzymes
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| - | available to us for carbohydrate biotransformations,6–13 we
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| - | were drawn to consider a-rhamnosidases.
| + | |
| - | In the CAZy database,14 rhamnosidases are currently
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| - | classified into GH28 pectin hydrolases, GH78 containing
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| - | exclusively rhamnosidases, and GH106 containing a single enzyme from Sphingomonas
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| - | == Disease ==
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| - | == Relevance ==
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| - | == Structural highlights ==
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| - | This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.
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| | </StructureSection> | | </StructureSection> |
| - | == References == | |
| - | <references/> | |
== Crystal structure of a novel two domain
GH78 family a-rhamnosidase from Klebsiella
oxytoca with rhamnose bound ==
