Sandbox viralpackagingmotors

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<Structure load='1W44' size='350' frame='true' align='right' caption='P4 Protein from Bacteriophage PH12 in complex with ADP [[1w44]]' />
<Structure load='1W44' size='350' frame='true' align='right' caption='P4 Protein from Bacteriophage PH12 in complex with ADP [[1w44]]' />
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One of the most important stage in the life cycle of all viruses is the encapsidation (packaging) of the viral genome. The viral genome packaging motor is a multicomponent molecular machine that must complete several task in sequential order, the foremost of which is the ATP-dependent pumping viral DNA into the procapsid.
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One of the most important stage in the life cycle of all viruses is the encapsidation (packaging) of the viral genome. Many virus package their genome into preformed capsids using packaging motors powered by the hydrolysis of ATP. The hexameric ATPase P4 of dsRNA bacteriophage phi 12, located at the vertices of the icosahedral capsid is such a packaging motor.
<ref name="rna packaging motor"> doi:10.1016/j.cell.2004.09.007</ref>
<ref name="rna packaging motor"> doi:10.1016/j.cell.2004.09.007</ref>
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Many viruses such as tailed dsDNA bacteriophages among others package their genome into empty capsids via a portal vertex complex. The packaging motor is generally multiple copies of a single protein, and an example is the P4 protein that uses the RNA packaging motor. The RNA packaging motor is powered by the hydrolysis of ATP.
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Viruses like tailed dsDNA bacteriophages among others package their genome into empty capsids via a portal vertex complex. The packaging motor is generally multiple copies of a single protein, and an example is the P4 protein that uses the RNA packaging motor. The RNA packaging motor is powered by the hydrolysis of ATP.
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== Structural highlights ==
== Structural highlights ==
[[Image:1W44 cartoon.png|thumb|PDB 1W44 Atomic Snapshots of an RNA Packaging motor. Arg residue highlighted in magenta]]
[[Image:1W44 cartoon.png|thumb|PDB 1W44 Atomic Snapshots of an RNA Packaging motor. Arg residue highlighted in magenta]]
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This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.
This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.

Revision as of 18:03, 11 December 2015

This page is setup for Ojuewa to build her senior project for OU CHEM 4923

Viral Packaging

P4 Protein from Bacteriophage PH12 in complex with ADP 1w44

Drag the structure with the mouse to rotate

One of the most important stage in the life cycle of all viruses is the encapsidation (packaging) of the viral genome. Many virus package their genome into preformed capsids using packaging motors powered by the hydrolysis of ATP. The hexameric ATPase P4 of dsRNA bacteriophage phi 12, located at the vertices of the icosahedral capsid is such a packaging motor. [1] Viruses like tailed dsDNA bacteriophages among others package their genome into empty capsids via a portal vertex complex. The packaging motor is generally multiple copies of a single protein, and an example is the P4 protein that uses the RNA packaging motor. The RNA packaging motor is powered by the hydrolysis of ATP.


Contents

Function

The RNA packaging motor is a straightforward machinery that consists of a portal protein (P4) hexamer. It is the P4 protein that provides energy for RNA translocation.

Disease

The two methods of virion nucleic acid packaging known are (1) co-condensation of the nucleic acid with viral capsid proteins to form a virus particle ( employed by viruses such as HIV, TMV, AND M13 and (2) translocation of the nucleic acid into the a preformed procapsid shell ( found in eukaryotic viral families such as herpes and tailed dsDNA bacteriophages such T4, lambda and phi29, among others). [2]

Relevance

Structural highlights

PDB 1W44 Atomic Snapshots of an RNA Packaging motor. Arg residue highlighted in magenta
PDB 1W44 Atomic Snapshots of an RNA Packaging motor. Arg residue highlighted in magenta


This is a sample scene created with SAT to by Group, and another to make of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.

</StructureSection>

References

  1. ↑ Mancini EJ, Kainov DE, Grimes JM, Tuma R, Bamford DH, Stuart DI. Atomic snapshots of an RNA packaging motor reveal conformational changes linking ATP hydrolysis to RNA translocation. Cell. 2004 Sep 17;118(6):743-55. PMID:15369673 doi:10.1016/j.cell.2004.09.007
  2. ↑ doi: https://dx.doi.org/10.1039/9781849732239-00203
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