4uw8

From Proteopedia

(Difference between revisions)

Revision as of 07:40, 16 December 2015

Structure of the carboxy-terminal domain of the bacteriophage T5 L- shaped tail fiber with its intra-molecular chaperone domain

Structural highlights

4uw8 is a 9 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	4uw7
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum

Function

[FIBL_BPT5] Assembles together with p132 to form the three L-shaped long tail fibers that recognize the host lipopolysaccharides that serve as adhesion receptor for virus entry. P132 makes the proximal part and pb8 the distal part that binds to the host cell surface. The L-shaped long tail fibers are attached to a collar structure at the junction between the tail tube and the conical tail tip. Each fiber consists of a thin proximal rod of about 30 nm connected by a hinge to a thicker distal part of about 47 nm.^[1] ^[2]

Publication Abstract from PubMed

Tails of bacteriophage T5 (a member of the Siphoviridae family) were studied by electron microscopy. For the distal parts of the L-shaped tail fibres, which are involved in host cell receptor binding, a low-resolution volume was calculated. Several C-terminal fragments of the fibre were expressed and purified. Crystals of two of them were obtained that belonged to space groups P63 and R32 and diffracted synchrotron radiation to 2.3 and 2.9 A resolution, respectively. A single-wavelength anomalous dispersion data set to 2.5 A resolution was also collected from a selenomethionine-derivatized crystal of one of the fragments, which belonged to space group C2.

Crystallization of the C-terminal domain of the bacteriophage T5 L-shaped fibre.,Garcia-Doval C, Luque D, Caston JR, Boulanger P, van Raaij MJ Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Dec;69(Pt 12):1363-7. doi:, 10.1107/S1744309113028959. Epub 2013 Nov 28. PMID:24316831^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zivanovic Y, Confalonieri F, Ponchon L, Lurz R, Chami M, Flayhan A, Renouard M, Huet A, Decottignies P, Davidson AR, Breyton C, Boulanger P. Insights into bacteriophage T5 structure from analysis of its morphogenesis genes and protein components. J Virol. 2014 Jan;88(2):1162-74. doi: 10.1128/JVI.02262-13. Epub 2013 Nov 6. PMID:24198424 doi:http://dx.doi.org/10.1128/JVI.02262-13
↑ Heller K, Braun V. Polymannose O-antigens of Escherichia coli, the binding sites for the reversible adsorption of bacteriophage T5+ via the L-shaped tail fibers. J Virol. 1982 Jan;41(1):222-7. PMID:7045389
↑ Garcia-Doval C, Luque D, Caston JR, Boulanger P, van Raaij MJ. Crystallization of the C-terminal domain of the bacteriophage T5 L-shaped fibre. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Dec;69(Pt 12):1363-7. doi:, 10.1107/S1744309113028959. Epub 2013 Nov 28. PMID:24316831 doi:http://dx.doi.org/10.1107/S1744309113028959

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4uw8"

@@ Line 5: / Line 5: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4uw7|4uw7]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4uw8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uw8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4uw8 RCSB], [http://www.ebi.ac.uk/pdbsum/4uw8 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4uw8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uw8 OCA], [http://pdbe.org/4uw8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4uw8 RCSB], [http://www.ebi.ac.uk/pdbsum/4uw8 PDBsum]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 17: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4uw8" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
@@ Line 24: / Line 25: @@
 [[Category: Caston, J R]]
 [[Category: Garcia-Doval, C]]
-[[Category: Llamas-Saizd, A L]]
+[[Category: Llamas-Saiz, A L]]
 [[Category: Luque, D]]
-[[Category: Oteroc, J M]]
+[[Category: Otero, J M]]
 [[Category: Raaij, M J.van]]
 [[Category: Bacterial viruse]]

4uw8

From Proteopedia

Revision as of 07:40, 16 December 2015

Structure of the carboxy-terminal domain of the bacteriophage T5 L- shaped tail fiber with its intra-molecular chaperone domain

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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