1auu

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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1auu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1auu OCA], [http://www.ebi.ac.uk/pdbsum/1auu PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1auu RCSB]</span>
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[[Category: transcription regulation]]
[[Category: transcription regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:47:57 2008''

Revision as of 15:47, 30 March 2008


PDB ID 1auu

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Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



SOLUTION STRUCTURE OF THE RNA-BINDING DOMAIN OF THE ANTITERMINATOR PROTEIN SACY, NMR, 10 STRUCTURES


Overview

SacY is the prototype of a family of regulatory proteins able to prevent transcription termination. It interacts with a 29 nucleotide RNA sequence able to fold into a stem-loop structure and partially overlapping with a terminator sequence located in the 5' leader mRNA region of the gene it controls. We show here that the N-terminal fragment of SacY, SacY(1-55), and the corresponding fragments of other members of the family have antiterminator activities with efficiency and specificity identical to those of the full-length proteins. In vitro, this activity correlates with the specific affinity of SacY(1-55) for its RNA target. UV melting experiments demonstrate that SacY(1-55) binding stabilizes the RNA target structure. The NMR solution structure of SacY(1-55) is very similar to that obtained in the crystal (van Tilbeurgh et al., 1997): the peptide is folded as a symmetrical dimer without any structural homology with other RNA-binding domains yet characterized. According to a preliminary NMR analysis of the SacY(1-55)-RNA complex, the protein dimer is not disrupted upon RNA binding and several residues implicated in RNA recognition are located at the edge of the dimer interface. This suggests a new mode of protein-RNA interaction.

About this Structure

1AUU is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

From genetic to structural characterization of a new class of RNA-binding domain within the SacY/BglG family of antiterminator proteins., Manival X, Yang Y, Strub MP, Kochoyan M, Steinmetz M, Aymerich S, EMBO J. 1997 Aug 15;16(16):5019-29. PMID:9305643

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