1aw2
From Proteopedia
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|PDB= 1aw2 |SIZE=350|CAPTION= <scene name='initialview01'>1aw2</scene>, resolution 2.65Å | |PDB= 1aw2 |SIZE=350|CAPTION= <scene name='initialview01'>1aw2</scene>, resolution 2.65Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | + | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1aw2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aw2 OCA], [http://www.ebi.ac.uk/pdbsum/1aw2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1aw2 RCSB]</span> | ||
}} | }} | ||
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[[Category: Wierenga, R K.]] | [[Category: Wierenga, R K.]] | ||
[[Category: Zeelen, J P.]] | [[Category: Zeelen, J P.]] | ||
| - | [[Category: SO4]] | ||
[[Category: isomerase]] | [[Category: isomerase]] | ||
[[Category: psychrophilic]] | [[Category: psychrophilic]] | ||
[[Category: vibrio marinus]] | [[Category: vibrio marinus]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:48:37 2008'' |
Revision as of 15:48, 30 March 2008
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| , resolution 2.65Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Activity: | Triose-phosphate isomerase, with EC number 5.3.1.1 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
TRIOSEPHOSPHATE ISOMERASE OF VIBRIO MARINUS
Overview
The purification and characterization of triose-phosphate isomerase from the psychrophilic bacterium Vibrio marinus (vTIM) is described. Crystal structures of the vTIM-sulfate complex and the vTIM-2-phosphoglycolate complex (at a 2.7-A resolution) are also presented. The optimal growth temperature of Vibrio marinus is 15 degrees C. Stability studies show that vTIM is an unstable protein with a half-life of only 10 min at 25 degrees C. The vTIM sequence is most closely related to the sequence of Escherichia coli TIM (eTIM) (66% identity), and several unique structural features described for eTIM are also seen in vTIM, but eTIM is considerably more stable. The Td values of vTIM and eTIM, determined by calorimetric studies, are 41 and 54 degrees C, respectively. Amino acid sequence comparison reveals that vTIM has an alanine in loop 8 (at position 238), whereas all other TIM sequences known to date have a serine. The vTIM mutant A238S was produced and characterized. Compared with wild type, the catalytic efficiency of the A238S mutant is somewhat reduced, and its stability is considerably increased.
About this Structure
1AW2 is a Single protein structure of sequence from Moritella marina. Full crystallographic information is available from OCA.
Reference
Triose-phosphate isomerase (TIM) of the psychrophilic bacterium Vibrio marinus. Kinetic and structural properties., Alvarez M, Zeelen JP, Mainfroid V, Rentier-Delrue F, Martial JA, Wyns L, Wierenga RK, Maes D, J Biol Chem. 1998 Jan 23;273(4):2199-206. PMID:9442062
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