1ax3

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|SITE= <scene name='pdbsite=PON:Phosphorylation+Site'>PON</scene>
|SITE= <scene name='pdbsite=PON:Phosphorylation+Site'>PON</scene>
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|LIGAND=
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|ACTIVITY= [http://en.wikipedia.org/wiki/Protein-N(pi)-phosphohistidine--sugar_phosphotransferase Protein-N(pi)-phosphohistidine--sugar phosphotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.69 2.7.1.69]
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-N(pi)-phosphohistidine--sugar_phosphotransferase Protein-N(pi)-phosphohistidine--sugar phosphotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.69 2.7.1.69] </span>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ax3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ax3 OCA], [http://www.ebi.ac.uk/pdbsum/1ax3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ax3 RCSB]</span>
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[[Category: transmembrane]]
[[Category: transmembrane]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:49:17 2008''

Revision as of 15:49, 30 March 2008


PDB ID 1ax3

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Sites:
Activity: Protein-N(pi)-phosphohistidine--sugar phosphotransferase, with EC number 2.7.1.69
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



SOLUTION NMR STRUCTURE OF B. SUBTILIS IIAGLC, 16 STRUCTURES


Overview

The high-resolution solution structure of the phosphocarrier protein IIAglc from Bacillus subtilis is determined using 3D and 4D heteronuclear NMR methods. B. subtilis IIAglc contains 162 amino acid residues and is one of the larger proteins for which high-resolution solution structure has been determined by NMR methods. The structures have been calculated from a total of 2,232 conformational constraints. Comparison with the X-ray crystal structure indicates that the overall fold is the same in solution and in crystalline environments, although some local structural differences are observed. These occur largely in turns and loops, and mostly correspond to regions with high-temperature factors in the crystal structure. The N-terminus of IIAglc is disordered in solution. The active site is located in a concave region of the protein surface. The histidine, which accepts the phosphoryl group (His 83), interacts with a neighboring histidine (His 68) and is surrounded by hydrophobic residues.

About this Structure

1AX3 is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

High-resolution solution structure of Bacillus subtilis IIAglc., Chen Y, Case DA, Reizer J, Saier MH Jr, Wright PE, Proteins. 1998 May 15;31(3):258-70. PMID:9593197

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