5ezk

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'''Unreleased structure'''
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==RNA polymerase model placed by Molecular replacement into X-ray diffraction map of DNA-bound RNA Polymerase-Sigma 54 holoenzyme complex.==
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<StructureSection load='5ezk' size='340' side='right' caption='[[5ezk]], [[Resolution|resolution]] 8.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5ezk]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EZK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EZK FirstGlance]. <br>
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</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ezk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ezk OCA], [http://pdbe.org/5ezk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ezk RCSB], [http://www.ebi.ac.uk/pdbsum/5ezk PDBsum]</span></td></tr>
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</table>
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== Function ==
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[[http://www.uniprot.org/uniprot/RPOZ_ECOLI RPOZ_ECOLI]] Promotes RNA polymerase assembly. Latches the N- and C-terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits.[HAMAP-Rule:MF_00366] [[http://www.uniprot.org/uniprot/RPOA_ECO57 RPOA_ECO57]] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. [[http://www.uniprot.org/uniprot/RPOC_ECO57 RPOC_ECO57]] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. [[http://www.uniprot.org/uniprot/RPOB_ECO57 RPOB_ECO57]] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Transcription by RNA polymerase (RNAP) in bacteria requires specific promoter recognition by sigma factors. The major variant sigma factor (sigma(54)) initially forms a transcriptionally silent complex requiring specialized adenosine triphosphate-dependent activators for initiation. Our crystal structure of the 450-kilodalton RNAP-sigma(54) holoenzyme at 3.8 angstroms reveals molecular details of sigma(54) and its interactions with RNAP. The structure explains how sigma(54) targets different regions in RNAP to exert its inhibitory function. Although sigma(54) and the major sigma factor, sigma(70), have similar functional domains and contact similar regions of RNAP, unanticipated differences are observed in their domain arrangement and interactions with RNAP, explaining their distinct properties. Furthermore, we observe evolutionarily conserved regulatory hotspots in RNAPs that can be targeted by a diverse range of mechanisms to fine tune transcription.
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The entry 5ezk is ON HOLD
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TRANSCRIPTION. Structures of the RNA polymerase-sigma54 reveal new and conserved regulatory strategies.,Yang Y, Darbari VC, Zhang N, Lu D, Glyde R, Wang YP, Winkelman JT, Gourse RL, Murakami KS, Buck M, Zhang X Science. 2015 Aug 21;349(6250):882-5. doi: 10.1126/science.aab1478. PMID:26293966<ref>PMID:26293966</ref>
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Authors: Darbari, V.C., Yang, Y., Lu, D., Zhang, N., Glyde, R., Wang, Y., Murakami, K.S., Buck, M., Zhang, X.
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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Description: RNA polymerase model placed by Molecular replacement into X-ray diffraction map of DNA-bound RNA Polymerase-Sigma 54 holoenzyme complex.
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<div class="pdbe-citations 5ezk" style="background-color:#fffaf0;"></div>
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[[Category: Unreleased Structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: DNA-directed RNA polymerase]]
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[[Category: Buck, M]]
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[[Category: Darbari, V C]]
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[[Category: Glyde, R]]
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[[Category: Lu, D]]
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[[Category: Murakami, K S]]
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[[Category: Wang, Y]]
[[Category: Yang, Y]]
[[Category: Yang, Y]]
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[[Category: Darbari, V.C]]
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[[Category: Zhang, N]]
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[[Category: Wang, Y]]
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[[Category: Zhang, X]]
[[Category: Zhang, X]]
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[[Category: Murakami, K.S]]
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[[Category: Rna polymerase]]
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[[Category: Zhang, N]]
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[[Category: Transferase]]
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[[Category: Glyde, R]]
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[[Category: Lu, D]]
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[[Category: Buck, M]]
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Revision as of 13:50, 16 December 2015

RNA polymerase model placed by Molecular replacement into X-ray diffraction map of DNA-bound RNA Polymerase-Sigma 54 holoenzyme complex.

5ezk, resolution 8.50Å

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