Sandbox viralpackagingmotors

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One of the most important stage in the life cycle of all viruses is the encapsidation (packaging) of the viral genome. Many virus package their genome into preformed capsids using packaging motors powered by the hydrolysis of ATP. The hexameric ATPase P4 of dsRNA bacteriophage phi 12, located at the vertices of the icosahedral capsid is such a packaging motor.
One of the most important stage in the life cycle of all viruses is the encapsidation (packaging) of the viral genome. Many virus package their genome into preformed capsids using packaging motors powered by the hydrolysis of ATP. The hexameric ATPase P4 of dsRNA bacteriophage phi 12, located at the vertices of the icosahedral capsid is such a packaging motor.
<ref name="rna packaging motor"> doi:10.1016/j.cell.2004.09.007</ref>
<ref name="rna packaging motor"> doi:10.1016/j.cell.2004.09.007</ref>
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P4 protein is a 35-kDa ssRNA packaging ATPase
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P4 protein is a 35-kDa ssRNA packaging ATPase from dsRNA bacteriophages belonging to the ''Cystoviridae'' family. ( phi 6 - phi 12). These viruses use a packaging motor like RNA packaging motor that is powered by the hydrolysis of ATP to condense the nucleic acid into a confined space.
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Viruses like tailed dsDNA bacteriophages among others package their genome into empty capsids via a portal vertex complex. The RNA packaging motor is powered by the hydrolysis of ATP.
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== Function ==
== Function ==
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The RNA packaging motor is a straightforward machinery that consists of a portal protein (P4) hexamer. It is the P4 protein that provides energy for RNA translocation.
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The RNA packaging motor is a straightforward machinery that consists of a portal protein (P4) hexamer.
== Energetics ==
== Energetics ==
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The P4 protein provides energy for the RNA translocation.
== Structural highlights ==
== Structural highlights ==

Revision as of 15:34, 16 December 2015

This page is setup for Ojuewa to build her senior project for OU CHEM 4923

Contents

Viral Packaging

P4 Protein from Bacteriophage PH12 in complex with ADP 1w44

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One of the most important stage in the life cycle of all viruses is the encapsidation (packaging) of the viral genome. Many virus package their genome into preformed capsids using packaging motors powered by the hydrolysis of ATP. The hexameric ATPase P4 of dsRNA bacteriophage phi 12, located at the vertices of the icosahedral capsid is such a packaging motor. [1] P4 protein is a 35-kDa ssRNA packaging ATPase from dsRNA bacteriophages belonging to the Cystoviridae family. ( phi 6 - phi 12). These viruses use a packaging motor like RNA packaging motor that is powered by the hydrolysis of ATP to condense the nucleic acid into a confined space.


Function

The RNA packaging motor is a straightforward machinery that consists of a portal protein (P4) hexamer.

Energetics

The P4 protein provides energy for the RNA translocation.

Structural highlights

PDB 1W44 Atomic Snapshots of an RNA Packaging motor. Arg residue highlighted in magenta
PDB 1W44 Atomic Snapshots of an RNA Packaging motor. Arg residue highlighted in magenta


This is a sample scene created with SAT to by Group, and another to make of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.

</StructureSection>

References

  1. ↑ Mancini EJ, Kainov DE, Grimes JM, Tuma R, Bamford DH, Stuart DI. Atomic snapshots of an RNA packaging motor reveal conformational changes linking ATP hydrolysis to RNA translocation. Cell. 2004 Sep 17;118(6):743-55. PMID:15369673 doi:10.1016/j.cell.2004.09.007
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