4zkt
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | ''' | + | ==Crystal structure of the progenitor M complex of Clostridium botulinum type E neurotoxin== |
| + | <StructureSection load='4zkt' size='340' side='right' caption='[[4zkt]], [[Resolution|resolution]] 3.05Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4zkt]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum_(strain_alaska_e43_/_type_e3) Clostridium botulinum (strain alaska e43 / type e3)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZKT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZKT FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zkt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zkt OCA], [http://pdbe.org/4zkt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zkt RCSB], [http://www.ebi.ac.uk/pdbsum/4zkt PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Clostridium botulinum neurotoxin (BoNT) is released as a progenitor complex, in association with a non-toxic-non-hemagglutinin protein (NTNH) and other associated proteins. We have determined the crystal structure of M type Progenitor complex of botulinum neurotoxin E [PTC-E(M)], a heterodimer of BoNT and NTNH. The crystal structure reveals that the complex exists as a tight, interlocked heterodimer of BoNT and NTNH. The crystal structure explains the mechanism of molecular assembly of the complex and reveals several acidic clusters at the interface responsible for association at low acidic pH and disassociation at basic/neutral pH. The similarity of the general architecture between the PTC-E(M) and the previously determined PTC-A(M) strongly suggests that the progenitor M complexes of all botulinum serotypes may have similar molecular arrangement, although the neurotoxins apparently can take very different conformation when they are released from the M complex. | ||
| - | + | Molecular Assembly of Clostridium botulinum progenitor M complex of type E.,Eswaramoorthy S, Sun J, Li H, Singh BR, Swaminathan S Sci Rep. 2015 Dec 7;5:17795. doi: 10.1038/srep17795. PMID:26639353<ref>PMID:26639353</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 4zkt" style="background-color:#fffaf0;"></div> | |
| - | [[Category: | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Bontoxilysin]] | ||
| + | [[Category: Eswaramoorthy, S]] | ||
[[Category: Swaminathan, S]] | [[Category: Swaminathan, S]] | ||
| - | [[Category: | + | [[Category: Acidic cluster]] |
| + | [[Category: Bont/e-ntnhe hetero-dimer]] | ||
| + | [[Category: Domain swap]] | ||
| + | [[Category: Hydrolase-toxin complex]] | ||
| + | [[Category: Progenitor complex]] | ||
Revision as of 12:43, 23 December 2015
Crystal structure of the progenitor M complex of Clostridium botulinum type E neurotoxin
| |||||||||||
