DNA ligase
From Proteopedia
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<StructureSection load='2hix' size='400' side='right' scene='DNA_ligase/Cv/1' caption='ATP-dependent DNA ligase complexed with ATP [[2hix]]'> | <StructureSection load='2hix' size='400' side='right' scene='DNA_ligase/Cv/1' caption='ATP-dependent DNA ligase complexed with ATP [[2hix]]'> | ||
| - | '''DNA ligase''' (LigD) is an enzyme which repairs single-stranded breaks in a double-stranded DNA. LigD is activated , in a species-dependent manner, by hydrolysis of ATP or NAD+. | + | '''DNA ligase''' (LigD) is an enzyme which repairs single-stranded breaks in a double-stranded DNA. LigD is activated, in a species-dependent manner, by hydrolysis of ATP or NAD+.<br /> |
| + | * Mammalian '''LigD I''' ligates the nascent DNA of the lagging strand.<br /> | ||
| + | * '''LigD III''' complexes with XRCC1 in the process of nucleotide excision repair.<br /> | ||
| + | * '''LigD IV''' complexes with XRCC4 and catalyzes the last step in the non-homologous DNA end joining.<br /> | ||
| + | See more details in [[ATP-Dependent DNA Ligase (Bacteriophage T7)]]. | ||
==Disease== | ==Disease== | ||
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A specific point mutation (46BR in the mouse Lig1 gene) in DNA ligase I has also been linked as a cause for genome instability and cancer in humans. One possible reason for this is the accumulation of DNA fragments that are no longer ligated by the mutant DNA ligase I enzyme. | A specific point mutation (46BR in the mouse Lig1 gene) in DNA ligase I has also been linked as a cause for genome instability and cancer in humans. One possible reason for this is the accumulation of DNA fragments that are no longer ligated by the mutant DNA ligase I enzyme. | ||
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| + | == Structural highlights == | ||
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| + | LigD contains several domains: adenylation domain; DNA-binding domain and the ca. 100 amino acid long BRCT motif. | ||
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</StructureSection> | </StructureSection> | ||
Revision as of 12:58, 31 December 2015
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3D Structures of DNA ligase
Updated on 31-December-2015
REFERENCES
Elevated expression of DNA ligase I in human cancers., Sun DY, Urrabaz R, Nguyen M, Marty J, Stringer S, Cruz E, Medina-Gundrum L, Weitman S., Clinical Cancer Research. 2001; 7(12):4143-4148.
Replication failure, genome instability, and increased cancer susceptibility in mice with a point mutation in the DNA ligase I gene., Harrison C, Ketchen AM, Redhead NJ, O'Sullivan MJ, Melton DW., Cancer Research. 2002; 62(14):4065-4074.
- Created with the participation of Audrey Kuan.
