Dronpa

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=== Structural highlights===
=== Structural highlights===
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The amino acids that are key for this change - forming spontaneously the <scene name='33/330991/Cv/5'>CYG bicyclic chromophore - are Cys 62, Tyr 63, and Gly 64</scene>. There are also changes in conformation of four additional amino acids that are near the chromophore, <scene name='33/330991/Cv/6'>Arg 66, Ser 142, Val 157, and His 193</scene>. <scene name='33/330991/Cv/6'>Click here to see all contacts of chromophore</scene>. <ref> Biochem J. (2007) 402, 35-42 </ref>.
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The amino acids that are key for this change - forming spontaneously the <scene name='33/330991/Cv/5'>CYG bicyclic chromophore - are Cys 62, Tyr 63, and Gly 64</scene>. There are also changes in conformation of four additional amino acids that are near the chromophore, <scene name='33/330991/Cv/6'>Arg 66, Ser 142, Val 157, and His 193</scene>. <scene name='33/330991/Cv/6'>Click here to see all contacts of chromophore</scene>. <ref>PMID:17117927</ref>
</StructureSection>
</StructureSection>
==3D structures of Dronpa==
==3D structures of Dronpa==

Revision as of 18:04, 5 January 2016

Fluorescent protein Dronpa complex with CYG chromophore 2iov

Drag the structure with the mouse to rotate

3D structures of Dronpa

Updated on 05-January-2016

2ie2, 2iov, 2gx0, 2gx2, 3zuf, 3zuj, 3zul – EcFPD – Echinophyllia
4izn - EcFDP (mutant)
2z1o, 2z6y, 2z6z – EcFDP bright state
2pox – EcFDP dark state
4hq8, 4emq - EcFDP green-on state (mutant)
4hq9 - EcFDP green-off state (mutant)
4hqc - EcFDP red state (mutant)
2z6x – FDP - Pectiniidae

Additional Resources

For additional information, see: Colored & Bioluminescent Proteins


References

  1. 1.0 1.1 [1] (retrieved on November 30, 2008),
  2. Stiel AC, Trowitzsch S, Weber G, Andresen M, Eggeling C, Hell SW, Jakobs S, Wahl MC. 1.8 A bright-state structure of the reversibly switchable fluorescent protein Dronpa guides the generation of fast switching variants. Biochem J. 2007 Feb 15;402(1):35-42. PMID:17117927 doi:10.1042/BJ20061401

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Michal Harel, Alexander Berchansky, Jamie LaFrance, David Canner

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