1bi6
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
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| + | |RELATEDENTRY=[[2bi6|2BI6]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bi6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bi6 OCA], [http://www.ebi.ac.uk/pdbsum/1bi6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1bi6 RCSB]</span> | ||
}} | }} | ||
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[[Category: cysteine protease inhibitor]] | [[Category: cysteine protease inhibitor]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:01:35 2008'' |
Revision as of 16:01, 30 March 2008
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| Related: | 2BI6
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
NMR STRUCTURE OF BROMELAIN INHIBITOR VI FROM PINEAPPLE STEM
Overview
Bromelain inhibitor VI from pineapple stem (BI-VI) is a unique double-chain inhibitor with an 11-residue light chain and a 41-residue heavy chain by disulfide bonds and inhibits the cysteine proteinase bromelain competitively. The structure of BI-VI in aqueous solution was determined using nuclear magnetic resonance spectroscopy and simulated annealing-based calculations. Its three-dimensional structure was shown to be composed of two distinct domains, each of which is formed by a three-stranded antiparallel beta-sheet. Unexpectedly, BI-VI was found to share a similar folding and disulfide bond connectivities not with cystatin superfamily inhibitors which inhibit the same cysteine proteinases but with the Bowman-Birk trypsin/chymotrypsin inhibitor from soybean (BBI-I). BBI-I is a 71-residue inhibitor which has two independent inhibitory sites toward the serine proteinases trypsin and chymotrypsin. These structural similarities with BBI-I suggest that they have evolved from a common ancestor and differentiated in function during a course of molecular evolution.
About this Structure
1BI6 is a Protein complex structure of sequences from Ananas comosus. Full crystallographic information is available from OCA.
Reference
Solution structure of bromelain inhibitor IV from pineapple stem: structural similarity with Bowman-Birk trypsin/chymotrypsin inhibitor from soybean., Hatano K, Kojima M, Tanokura M, Takahashi K, Biochemistry. 1996 Apr 30;35(17):5379-84. PMID:8611527
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