5flz

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'''Unreleased structure'''
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==Cryo-EM structure of gamma-TuSC oligomers in a closed conformation==
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<StructureSection load='5flz' size='340' side='right' caption='[[5flz]], [[Resolution|resolution]] 6.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5flz]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FLZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FLZ FirstGlance]. <br>
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</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5fm1|5fm1]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5flz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5flz OCA], [http://pdbe.org/5flz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5flz RCSB], [http://www.ebi.ac.uk/pdbsum/5flz PDBsum]</span></td></tr>
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</table>
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{{Large structure}}
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== Function ==
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[[http://www.uniprot.org/uniprot/SPC97_YEAST SPC97_YEAST]] Involved in microtubule organization by the microtubule organizing center, the spindle pole body (SPB). Probably part of the microtubule attachment site at the SPB. [[http://www.uniprot.org/uniprot/TBG_YEAST TBG_YEAST]] Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome, suggesting that it is involved in the minus-end nucleation of microtubule assembly. TUB4 is an important spindle pole body component that organizes both cytoplasmic and nuclear microtubule arrays. [[http://www.uniprot.org/uniprot/SPC98_YEAST SPC98_YEAST]] Involved in microtubule organization by the microtubule organizing center, the spindle pole body (SPB). Probably part of the microtubule attachment site at the SPB.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The gamma-tubulin ring complex (gammaTuRC) is the primary microtubule nucleator in cells. gammaTuRC is assembled from repeating gamma-tubulin small complex (gammaTuSC) subunits and is thought to function as a template by presenting a gamma-tubulin ring that mimics microtubule geometry. However, a previous yeast gammaTuRC structure showed gammaTuSC in an open conformation that prevents matching to microtubule symmetry. By contrast, we show here that gamma-tubulin complexes are in a closed conformation when attached to microtubules. To confirm the functional importance of the closed gammaTuSC ring, we trapped the closed state and determined its structure, showing that the gamma-tubulin ring precisely matches microtubule symmetry and providing detailed insight into gammaTuRC architecture. Importantly, the closed state is a stronger nucleator, thus suggesting that this conformational switch may allosterically control gammaTuRC activity. Finally, we demonstrate that gammaTuRCs have a strong preference for tubulin from the same species.
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The entry 5flz is ON HOLD
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Ring closure activates yeast gammaTuRC for species-specific microtubule nucleation.,Kollman JM, Greenberg CH, Li S, Moritz M, Zelter A, Fong KK, Fernandez JJ, Sali A, Kilmartin J, Davis TN, Agard DA Nat Struct Mol Biol. 2015 Feb;22(2):132-7. doi: 10.1038/nsmb.2953. Epub 2015 Jan , 19. PMID:25599398<ref>PMID:25599398</ref>
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Authors: Greenberg, C.H., Kollman, J., Zelter, A., Johnson, R., MacCoss, M.J., Davis, T.N., Agard, D.A., Sali, A.
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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Description: Cryo-EM structure of gamma-TuSC oligomers in a closed conformation
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<div class="pdbe-citations 5flz" style="background-color:#fffaf0;"></div>
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[[Category: Unreleased Structures]]
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== References ==
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[[Category: Greenberg, C.H]]
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<references/>
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[[Category: Zelter, A]]
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__TOC__
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[[Category: Sali, A]]
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</StructureSection>
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[[Category: Agard, D.A]]
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[[Category: Agard, D A]]
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[[Category: Maccoss, M.J]]
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[[Category: Davis, T N]]
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[[Category: Davis, T.N]]
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[[Category: Greenberg, C H]]
[[Category: Johnson, R]]
[[Category: Johnson, R]]
[[Category: Kollman, J]]
[[Category: Kollman, J]]
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[[Category: MacCoss, M J]]
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[[Category: Sali, A]]
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[[Category: Zelter, A]]
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[[Category: Cell cycle]]
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[[Category: Microtubule nucleation]]

Revision as of 20:04, 13 January 2016

Cryo-EM structure of gamma-TuSC oligomers in a closed conformation

5flz, resolution 6.90Å

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