1bos
From Proteopedia
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|PDB= 1bos |SIZE=350|CAPTION= <scene name='initialview01'>1bos</scene>, resolution 2.8Å | |PDB= 1bos |SIZE=350|CAPTION= <scene name='initialview01'>1bos</scene>, resolution 2.8Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene> | + | |LIGAND= <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=GLC:GLUCOSE'>GLC</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bos OCA], [http://www.ebi.ac.uk/pdbsum/1bos PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1bos RCSB]</span> | ||
}} | }} | ||
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[[Category: Ling, H.]] | [[Category: Ling, H.]] | ||
[[Category: Read, R J.]] | [[Category: Read, R J.]] | ||
| - | [[Category: GAL]] | ||
[[Category: ob-fold]] | [[Category: ob-fold]] | ||
[[Category: protein-carbohydrate recognition]] | [[Category: protein-carbohydrate recognition]] | ||
| Line 35: | Line 37: | ||
[[Category: toxin]] | [[Category: toxin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:05:13 2008'' |
Revision as of 16:05, 30 March 2008
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| , resolution 2.8Å | |||||||
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| Ligands: | , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
SHIGA-LIKE TOXIN COMPLEXED WITH ITS RECEPTOR
Overview
Shiga-like toxin I (SLT-I) is a virulence factor of Escherichia coli strains that cause disease in humans. Like other members of the Shiga toxin family, it consists of an enzymatic (A) subunit and five copies of a binding subunit (the B-pentamer). The B-pentamer binds to a specific glycolipid, globotriaosylceramide (Gb3), on the surface of target cells and thereby plays a crucial role in the entry of the toxin. Here we present the crystal structure at 2.8 A resolution of the SLT-I B-pentamer complexed with an analogue of the Gb3 trisaccharide. The structure reveals a surprising density of binding sites, with three trisaccharide molecules bound to each B-subunit monomer of 69 residues. All 15 trisaccharides bind to one side of the B-pentamer, providing further evidence that this side faces the cell membrane. The structural model is consistent with data from site-directed mutagenesis and binding of carbohydrate analogues, and allows the rational design of therapeutic Gb3 analogues that block the attachment of toxin to cells.
About this Structure
1BOS is a Single protein structure of sequence from Bacteriophage h30, bacteriophage h19b. Full crystallographic information is available from OCA.
Reference
Structure of the shiga-like toxin I B-pentamer complexed with an analogue of its receptor Gb3., Ling H, Boodhoo A, Hazes B, Cummings MD, Armstrong GD, Brunton JL, Read RJ, Biochemistry. 1998 Feb 17;37(7):1777-88. PMID:9485303
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