4v1k
From Proteopedia
(Difference between revisions)
Line 1: | Line 1: | ||
- | ''' | + | ==SeMet structure of a novel carbohydrate binding module from glycoside hydrolase family 9 (Cel9A) from Ruminococcus flavefaciens FD-1== |
+ | <StructureSection load='4v1k' size='340' side='right' caption='[[4v1k]], [[Resolution|resolution]] 1.60Å' scene=''> | ||
+ | == Structural highlights == | ||
+ | <table><tr><td colspan='2'>[[4v1k]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V1K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4V1K FirstGlance]. <br> | ||
+ | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2PE:NONAETHYLENE+GLYCOL'>2PE</scene>, <scene name='pdbligand=BGQ:2-HYDROXY+BUTANE-1,4-DIOL'>BGQ</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene></td></tr> | ||
+ | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
+ | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4v17|4v17]], [[4v18|4v18]], [[4v1b|4v1b]], [[4v1i|4v1i]], [[4v1l|4v1l]]</td></tr> | ||
+ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4v1k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v1k OCA], [http://pdbe.org/4v1k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4v1k RCSB], [http://www.ebi.ac.uk/pdbsum/4v1k PDBsum]</span></td></tr> | ||
+ | </table> | ||
+ | <div style="background-color:#fffaf0;"> | ||
+ | == Publication Abstract from PubMed == | ||
+ | Anaerobic bacteria organize carbohydrate-active enzymes into a multi-component complex, the cellulosome, which degrades cellulose and hemicellulose highly efficiently. Genome sequencing of Ruminococcus flavefaciens FD-1 offers extensive information on the range and diversity of the enzymatic and structural components of the cellulosome. The R. flavefaciens FD-1 genome encodes over 200 dockerin-containing proteins, most of which are of unknown function. One of these modular proteins comprises a glycoside hydrolase family 5 catalytic module (GH5) linked to an unclassified carbohydrate-binding module (CBM-Rf1) and a dockerin. The novel CBM-Rf1 has been purified and crystallized. The crystals belonged to the trigonal space group R32:H. The CBM-Rf1 structure was determined by a multiple-wavelength anomalous dispersion experiment using AutoSol from the PHENIX suite using both selenomethionyl-derivative and native data to resolutions of 2.28 and 2.0 A, respectively. | ||
- | + | Expression, purification and crystallization of a novel carbohydrate-binding module from the Ruminococcus flavefaciens cellulosome.,Venditto I, Centeno MS, Ferreira LM, Fontes CM, Najmudin S Acta Crystallogr F Struct Biol Commun. 2014 Dec 1;70(Pt 12):1653-6. doi:, 10.1107/S2053230X14024248. Epub 2014 Nov 14. PMID:25484220<ref>PMID:25484220</ref> | |
- | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
- | + | </div> | |
- | + | <div class="pdbe-citations 4v1k" style="background-color:#fffaf0;"></div> | |
- | + | == References == | |
- | [[Category: Ferreira, L | + | <references/> |
- | [[Category: | + | __TOC__ |
+ | </StructureSection> | ||
+ | [[Category: Ferreira, L M.A]] | ||
+ | [[Category: Fontes, C M.G A]] | ||
[[Category: Goyal, A]] | [[Category: Goyal, A]] | ||
[[Category: Najmudin, S]] | [[Category: Najmudin, S]] | ||
+ | [[Category: Thompson, A]] | ||
[[Category: Venditto, I]] | [[Category: Venditto, I]] | ||
- | [[Category: | + | [[Category: Carbohydrate binding module]] |
+ | [[Category: Cel9a]] | ||
+ | [[Category: Cellulosome]] | ||
+ | [[Category: Glycoside hydrolase family 9]] | ||
+ | [[Category: Ruminococcus flavefaciens fd-1]] | ||
+ | [[Category: Sugar binding protein]] |
Revision as of 16:55, 20 January 2016
SeMet structure of a novel carbohydrate binding module from glycoside hydrolase family 9 (Cel9A) from Ruminococcus flavefaciens FD-1
|