5fgp
From Proteopedia
(Difference between revisions)
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| - | ''' | + | ==Crystal structure of D. melanogaster Pur-alpha repeat I-II in complex with DNA.== |
| + | <StructureSection load='5fgp' size='340' side='right' caption='[[5fgp]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5fgp]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FGP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FGP FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fgp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fgp OCA], [http://pdbe.org/5fgp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fgp RCSB], [http://www.ebi.ac.uk/pdbsum/5fgp PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The neuronal DNA-/RNA-binding protein Pur-alpha is a transcription regulator and core factor for mRNA-localization. Pur-alpha deficient mice die after birth with pleiotropic neuronal defects. Here we report the crystal structure of the DNA-/RNA-binding domain of Pur-alpha in complex with ssDNA. It reveals base-specific recognition and offers a molecular explanation for the effect of point mutations in the 5q31.3 microdeletion syndrome. Consistent with the crystal structure, biochemical and NMR data indicate that Pur-alpha binds DNA and RNA in the same way, suggesting binding modes for tri- and hexanucleotide repeat RNAs in two neurodegenerative RNAopathies. Additionally, structure-based in vitro experiments resolved the molecular mechanism of Pur-alpha's unwindase activity. Complementing in vivo analyses in Drosophila demonstrated the importance of a highly conserved phenylalanine for Pur-alpha's unwinding and neuroprotective function. By uncovering the molecular mechanisms of nucleic-acid binding, this study contributes to understanding the cellular role of Pur-alpha and its implications in neurodegenerative diseases. | ||
| - | + | Structural basis of nucleic-acid recognition and double-strand unwinding by the essential neuronal protein Pur-alpha.,Weber J, Bao H, Hartlmuller C, Wang Z, Windhager A, Janowski R, Madl T, Jin P, Niessing D Elife. 2016 Jan 8;5. pii: e11297. doi: 10.7554/eLife.11297. PMID:26744780<ref>PMID:26744780</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 5fgp" style="background-color:#fffaf0;"></div> | |
| - | [[Category: | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Janowski, R]] | ||
| + | [[Category: Niessing, D]] | ||
[[Category: Weber, J]] | [[Category: Weber, J]] | ||
| - | [[Category: | + | [[Category: 5q31 3 microdeletion syndrome]] |
| - | [[Category: | + | [[Category: Al]] |
| + | [[Category: Dna binding protein]] | ||
| + | [[Category: Dna unwinding]] | ||
| + | [[Category: Dna-protein interaction]] | ||
| + | [[Category: Ftld]] | ||
| + | [[Category: Fxta]] | ||
| + | [[Category: Neurodegeneration]] | ||
| + | [[Category: Rna-protein interaction]] | ||
Revision as of 17:01, 20 January 2016
Crystal structure of D. melanogaster Pur-alpha repeat I-II in complex with DNA.
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