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Human FKBP52

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Revision as of 15:37, 22 January 2016

Crystal structure of N-terminus of human FKBP52 (1q1c)

(see also FK506 binding protein)

Publication Abstract from PubMed

FK506-binding protein 52 (FKBP52), which binds FK506 and possesses peptidylprolyl isomerase activity, is an important immunophilin involved in the heterocomplex of steroid receptors with heat-shock protein 90. Here we report the crystal structures of two overlapped fragments [N(1-260) and C(145-459)] of FKBP52 and the complex with a C-terminal pentapeptide from heat-shock protein 90. Based on the structures of these two overlapped fragments, the complete putative structure of FKBP52 can be defined. The structure of FKBP52 is composed of two consecutive FKBP domains, a tetratricopeptide repeat domain and a short helical domain beyond the final tetratricopeptide repeat motif. Key structural differences between FKBP52 and FKBP51, including the relative orientations of the four domains and some important residue substitutions, could account for the differential functions of FKBPs.

3D structure of human FK506-binding protein 52: implications for the assembly of the glucocorticoid receptor/Hsp90/immunophilin heterocomplex., Wu B, Li P, Liu Y, Lou Z, Ding Y, Shu C, Ye S, Bartlam M, Shen B, Rao Z, Proc Natl Acad Sci U S A. 2004 Jun 1;101(22):8348-53. Epub 2004 May 24. PMID:15159550

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

The 3D structures of several FKBP family members from various species are solved, most of them comprise 1-2 FK domains (e.g. human FKBP52), while wFKBP73 (e.g. 3jym) has 3 FK domains which is characteristic to plants. A sequence-based structure comparison between each of the 3 FK domains of wFKBP73 (3jym) and the 2 FK domains of hFKBP52 (1q1c) was performed. All 3 FK domains of wFKBP73 adopt a typical FK fold exhibiting significant diversity when superimposed. They are arranged in a linear manner in space as observed in the 2 FK domains of hFKBP52. While the 2 FK domains of hFKBP52 are in the same orientation, the orientation between any 2 consecutive wFK73 domains is different than that between the two FK domains of hFKBP52. of the wFK73_1 (in blueviolet) and wFK73_2 (in cyan) domains (3jym) on hFK52_1 (in yellow) and hFK52_2 (in blue) revealed that while wFK73_2 is perfectly aligned with hFK52_2, N-terminal wFK73_1 does not align with hFK52_1 (yellow). Similarly, of the wFK73_2 and wFK73_3 (in magenta) domains on hFKBP52 revealed that while wFK73_2 is perfectly aligned with hFK52_1 (in yellow), wFK73_3 does not align with hFK52_2. This unique arrangement of wFKBP73 causes that the α-helices of are exposed on the same surface, while the are presented on opposite surfaces.

About this Structure

1Q1C is a 1 chain structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

References

Wu B, Li P, Liu Y, Lou Z, Ding Y, Shu C, Ye S, Bartlam M, Shen B, Rao Z. 3D structure of human FK506-binding protein 52: implications for the assembly of the glucocorticoid receptor/Hsp90/immunophilin heterocomplex. Proc Natl Acad Sci U S A. 2004 Jun 1;101(22):8348-53. Epub 2004 May 24. PMID:15159550 doi:10.1073/pnas.0305969101
Unger T, Dym O, Albeck S, Jacobovitch Y, Bernehim R, Marom D, Pisanty O, Breiman A. Crystal structure of the three FK506 binding protein domains of wheat FKBP73: evidence for a unique wFK73_2 domain. J Struct Funct Genomics. 2010 Jun;11(2):113-23. Epub 2010 Mar 20. PMID:20306145 doi:10.1007/s10969-010-9085-8

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

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-	<StructureSection load='1q1c~~.pdb~~' size="350~~" color="" frame="true" spin="on" Scene~~='' ~~align~~=~~"right"~~ caption=' Human FKBP52 N-terminal [[1q1c]]' >	+	<StructureSection load='1q1c' size='350' side='right' scene='' caption='Human FKBP52 N-terminal (PDB code [[1q1c]]) '>
	[[Image:1q1c.png\|left\|200px]]		[[Image:1q1c.png\|left\|200px]]

Human FKBP52

From Proteopedia

Revision as of 15:37, 22 January 2016

Crystal structure of N-terminus of human FKBP52 (1q1c)

About this Structure

References

Proteopedia Page Contributors and Editors (what is this?)

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