1c9m
From Proteopedia
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|PDB= 1c9m |SIZE=350|CAPTION= <scene name='initialview01'>1c9m</scene>, resolution 1.67Å | |PDB= 1c9m |SIZE=350|CAPTION= <scene name='initialview01'>1c9m</scene>, resolution 1.67Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SEB:O-BENZYLSULFONYL-SERINE'>SEB</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1c13|1C13]], [[1c9j|1C9J]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1c9m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c9m OCA], [http://www.ebi.ac.uk/pdbsum/1c9m PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1c9m RCSB]</span> | ||
}} | }} | ||
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[[Category: Subtilisin]] | [[Category: Subtilisin]] | ||
[[Category: Bott, R.]] | [[Category: Bott, R.]] | ||
| - | [[Category: CA]] | ||
| - | [[Category: SO4]] | ||
[[Category: altered flexibility]] | [[Category: altered flexibility]] | ||
[[Category: serine protease]] | [[Category: serine protease]] | ||
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[[Category: subtilisin]] | [[Category: subtilisin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:17:27 2008'' |
Revision as of 16:17, 30 March 2008
| |||||||
| , resolution 1.67Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Activity: | Subtilisin, with EC number 3.4.21.62 | ||||||
| Related: | 1C13, 1C9J
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
BACILLUS LENTUS SUBTILSIN (SER 87) N76D/S103A/V104I
Overview
The three-dimensional structures of engineered variants of Bacillus lentus subtilisin having increased enzymatic activity, K27R/N87S/V104Y/N123S/T274A (RSYSA) and N76D/N87S/S103A/V104I (DSAI), were determined by X-ray crystallography. In addition to identifying changes in atomic position we report a method that identifies protein segments having altered flexibility. The method utilizes a statistical analysis of variance to delineate main-chain temperature factors that represent significant departures from the overall variance between equivalent regions seen throughout the structure. This method reveals changes in main-chain mobility in both variants. Residues 125-127 have increased mobility in the RSYSA variant while residues 100-104 have decreased mobility in the DSAI variant. These segments are located at the substrate-binding site and changes in their mobility are believed to relate to the observed changes in proteolytic activity. The effect of altered crystal lattice contacts on segment flexibility becomes apparent when identical variants, determined in two crystal forms, are compared with the native enzyme.
About this Structure
1C9M is a Single protein structure of sequence from Bacillus lentus. Full crystallographic information is available from OCA.
Reference
Engineered Bacillus lentus subtilisins having altered flexibility., Graycar T, Knapp M, Ganshaw G, Dauberman J, Bott R, J Mol Biol. 1999 Sep 10;292(1):97-109. PMID:10493860
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