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1c9s
From Proteopedia
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|PDB= 1c9s |SIZE=350|CAPTION= <scene name='initialview01'>1c9s</scene>, resolution 1.90Å | |PDB= 1c9s |SIZE=350|CAPTION= <scene name='initialview01'>1c9s</scene>, resolution 1.90Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene> | + | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1c9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c9s OCA], [http://www.ebi.ac.uk/pdbsum/1c9s PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1c9s RCSB]</span> | ||
}} | }} | ||
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[[Category: Gollnick, P.]] | [[Category: Gollnick, P.]] | ||
[[Category: Greaves, R B.]] | [[Category: Greaves, R B.]] | ||
| - | [[Category: TRP]] | ||
[[Category: protein-rna complex]] | [[Category: protein-rna complex]] | ||
[[Category: single stranded rna]] | [[Category: single stranded rna]] | ||
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[[Category: trap]] | [[Category: trap]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:17:31 2008'' |
Revision as of 16:17, 30 March 2008
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| , resolution 1.90Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF A COMPLEX OF TRP RNA-BINDING ATTENUATION PROTEIN WITH A 53-BASE SINGLE STRANDED RNA CONTAINING ELEVEN GAG TRIPLETS SEPARATED BY AU DINUCLEOTIDES
Overview
The trp RNA-binding attenuation protein (TRAP) regulates expression of the tryptophan biosynthetic genes of several bacilli by binding single-stranded RNA. The binding sequence is composed of eleven triplet repeats, predominantly GAG, separated by two or three non-conserved nucleotides. Here we present the crystal structure of a complex of TRAP and a 53-base single-stranded RNA containing eleven GAG triplets, revealing that each triplet is accommodated in a binding pocket formed by beta-strands. In the complex, the RNA has an extended structure without any base-pairing and binds to the protein mostly by specific protein-base interactions. Eleven binding pockets on the circular TRAP 11-mer form a belt with a diameter of about 80 A. This simple but elegant mechanism of arresting the RNA segment by encircling it around a protein disk is applicable to both transcription, when TRAP binds the nascent RNA, and to translation, when TRAP binds the same sequence within a non-coding leader region of the messenger RNA.
About this Structure
1C9S is a Single protein structure of sequence from Geobacillus stearothermophilus. Full crystallographic information is available from OCA.
Reference
Structure of the trp RNA-binding attenuation protein, TRAP, bound to RNA., Antson AA, Dodson EJ, Dodson G, Greaves RB, Chen X, Gollnick P, Nature. 1999 Sep 16;401(6750):235-42. PMID:10499579
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