1cl4
From Proteopedia
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|PDB= 1cl4 |SIZE=350|CAPTION= <scene name='initialview01'>1cl4</scene> | |PDB= 1cl4 |SIZE=350|CAPTION= <scene name='initialview01'>1cl4</scene> | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | + | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= NCP14 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= Simian Mason-Pfizer virus]) | |GENE= NCP14 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= Simian Mason-Pfizer virus]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cl4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cl4 OCA], [http://www.ebi.ac.uk/pdbsum/1cl4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1cl4 RCSB]</span> | ||
}} | }} | ||
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[[Category: Giedroc, D P.]] | [[Category: Giedroc, D P.]] | ||
[[Category: Kaluarachchi, K.]] | [[Category: Kaluarachchi, K.]] | ||
| - | [[Category: ZN]] | ||
[[Category: nucleocapsid protein]] | [[Category: nucleocapsid protein]] | ||
[[Category: retrovirus]] | [[Category: retrovirus]] | ||
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[[Category: viral protein]] | [[Category: viral protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:23:48 2008'' |
Revision as of 16:23, 30 March 2008
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| Ligands: | |||||||
| Gene: | NCP14 (Simian Mason-Pfizer virus) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
NUCLEOCAPSID PROTEIN FROM MASON-PFIZER MONKEY VIRUS (MPMV)
Overview
Retroviral nucleocapsid proteins (NCPs) are CCHC-type zinc finger proteins that mediate virion RNA binding activities associated with retrovirus assembly and genomic RNA encapsidation. Mason-Pfizer monkey virus (MPMV), a type D retrovirus, encodes a 96-amino acid nucleocapsid protein, which contains two Cys-X2-Cys-X4-His-X4-Cys (CCHC) zinc fingers connected by an unusually long 15-amino acid linker. Homonuclear, two-dimensional sensitivity-enhanced 15N-1H, three-dimensional 15N-1H, and triple resonance NMR spectroscopy have been used to determine the solution structure and residue-specific backbone dynamics of the structured core domain of MPMV NCP containing residues 21-80. Structure calculations and spectral density mapping of N-H bond vector mobility reveal that MPMV NCP 21-80 is best described as two independently folded, rotationally uncorrelated globular domains connected by a seven-residue flexible linker consisting of residues 42-48. The N-terminal CCHC zinc finger domain (residues 24-37) appears to adopt a fold like that described previously for HIV-1 NCP; however, residues within this domain and the immediately adjacent linker region (residues 38-41) are characterized by extensive conformational averaging on the micros-ms time scale at 25 degrees C. In contrast to other NCPs, residues 49-77, which includes the C-terminal CCHC zinc-finger (residues 53-66), comprise a well-folded globular domain with the Val49-Pro-Gly-Leu52 sequence and C-terminal tail residues 67-77 characterized by amide proton exchange properties and 15N R1, R2, and (1H-15N) NOE values indistinguishable to residues in the core C-terminal finger. Twelve refined structural models of MPMV NCP residues 49-80 (pairwise backbone RMSD of 0.77 A) reveal that the side chains of the conserved Pro50 and Trp62 are in van der Waals contact with one another. Residues 70-73 in the C-terminal tail adopt a reverse turn-like structure. Ile77 is involved in extensive van der Waals contact with the core finger domain, while the side chains of Ser68 and Asn75 appear to form hydrogen bonds that stabilize the overall fold of this domain. These residues outside of the core finger structure are conserved in D-type and related retroviral NCPs, e.g., MMTV NCP, suggesting that the structure of MPMV NCP may be representative of this subclass of retroviral NCPs.
About this Structure
1CL4 is a Single protein structure of sequence from Simian mason-pfizer virus. Full crystallographic information is available from OCA.
Reference
Solution structure and backbone dynamics of Mason-Pfizer monkey virus (MPMV) nucleocapsid protein., Gao Y, Kaluarachchi K, Giedroc DP, Protein Sci. 1998 Nov;7(11):2265-80. PMID:9827993
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