1cv9
From Proteopedia
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|PDB= 1cv9 |SIZE=350|CAPTION= <scene name='initialview01'>1cv9</scene> | |PDB= 1cv9 |SIZE=350|CAPTION= <scene name='initialview01'>1cv9</scene> | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene> | + | |LIGAND= <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cv9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cv9 OCA], [http://www.ebi.ac.uk/pdbsum/1cv9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1cv9 RCSB]</span> | ||
}} | }} | ||
| Line 25: | Line 28: | ||
[[Category: Harrison, M L.]] | [[Category: Harrison, M L.]] | ||
[[Category: Post, C B.]] | [[Category: Post, C B.]] | ||
| - | [[Category: ACE]] | ||
| - | [[Category: NH2]] | ||
[[Category: immunoreceptor tyrosine activation motif]] | [[Category: immunoreceptor tyrosine activation motif]] | ||
[[Category: itam]] | [[Category: itam]] | ||
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[[Category: peptide substate]] | [[Category: peptide substate]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:29:11 2008'' |
Revision as of 16:29, 30 March 2008
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| Ligands: | , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
NMR STUDY OF ITAM PEPTIDE SUBSTRATE
Overview
The immunoreceptor tyrosine-based activation motif (ITAM) plays a central role in transmembrane signal transduction in hematopoietic cells by mediating responses leading to proliferation and differentiation. An initial signaling event following activation of the B cell antigen receptor is phosphorylation of the CD79a (Ig-alpha) ITAM by Lyn, a Src family protein-tyrosine kinase. To elucidate the structural basis for recognition between the ITAM substrate and activated Lyn kinase, the structure of an ITAM-derived peptide bound to Lyn was determined using exchange-transferred nuclear Overhauser NMR spectroscopy. The bound substrate structure has an irregular helix-like character. Docking based on the NMR data into the active site of the closely related Lck kinase strongly favors ITAM binding in an orientation similar to binding of cyclic AMP-dependent protein kinase rather than that of insulin receptor tyrosine kinase. The model of the complex provides a rationale for conserved ITAM residues, substrate specificity, and suggests that substrate binds only the active conformation of the Src family tyrosine kinase, unlike the ATP cofactor, which can bind the inactive form.
About this Structure
1CV9 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Substrate recognition by the Lyn protein-tyrosine kinase. NMR structure of the immunoreceptor tyrosine-based activation motif signaling region of the B cell antigen receptor., Gaul BS, Harrison ML, Geahlen RL, Burton RA, Post CB, J Biol Chem. 2000 May 26;275(21):16174-82. PMID:10748115
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