1d7q
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
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| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1d7q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d7q OCA], [http://www.ebi.ac.uk/pdbsum/1d7q PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1d7q RCSB]</span> | ||
}} | }} | ||
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[[Category: rna-binding protein]] | [[Category: rna-binding protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:36:20 2008'' |
Revision as of 16:36, 30 March 2008
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
HUMAN TRANSLATION INITIATION FACTOR EIF1A
Overview
The translation initiation factor eIF1A is necessary for directing the 43S preinitiation complex from the 5' end of the mRNA to the initiation codon in a process termed scanning. We have determined the solution structure of human eIF1A, which reveals an oligonucleotide-binding (OB) fold and an additional domain. NMR titration experiments showed that eIF1A binds single-stranded RNA oligonucleotides in a site-specific, but non-sequence-specific manner, hinting at an mRNA interaction rather than specific rRNA or tRNA binding. The RNA binding surface extends over a large area covering the canonical OB fold binding site as well as a groove leading to the second domain. Site-directed mutations at multiple positions along the RNA-binding surface were defective in the ability to properly assemble preinitiation complexes at the AUG codon in vitro.
About this Structure
1D7Q is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The eIF1A solution structure reveals a large RNA-binding surface important for scanning function., Battiste JL, Pestova TV, Hellen CU, Wagner G, Mol Cell. 2000 Jan;5(1):109-19. PMID:10678173
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