1d8k
From Proteopedia
| Line 7: | Line 7: | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1d8j|1D8J]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1d8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d8k OCA], [http://www.ebi.ac.uk/pdbsum/1d8k PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1d8k RCSB]</span> | ||
}} | }} | ||
| Line 34: | Line 37: | ||
[[Category: winged helix-turn-helix]] | [[Category: winged helix-turn-helix]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:36:45 2008'' |
Revision as of 16:36, 30 March 2008
| |||||||
| Related: | 1D8J
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
SOLUTION STRUCTURE OF THE CENTRAL CORE DOMAIN OF TFIIE BETA
Overview
Human general transcription factor TFIIE consists of two subunits, TFIIEalpha and TFIIEbeta. Recently, TFIIEbeta has been found to bind to the region where the promoter starts to open to be single-stranded upon transcription initiation by RNA polymerase II. Here, the central core domain of human TFIIEbeta (TFIIEbetac) has been identified by a limited proteolysis. This solution structure has been determined by NMR. It consists of three helices with a beta hairpin at the C-terminus, resembling the winged helix proteins. However, TFIIEbetac shows a novel double-stranded DNA-binding activity where the DNA-binding surface locates on the opposite side to the previously reported winged helix motif by forming a positively charged furrow. A model will be proposed that TFIIE stabilizes the preinitiation complex by binding not only to the general transcription factors together with RNA polymerase II but also to the promoter DNA, where double-stranded DNA starts to open to be single-stranded upon activation of the preinitiation complex.
About this Structure
1D8K is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of the central core domain of TFIIEbeta with a novel double-stranded DNA-binding surface., Okuda M, Watanabe Y, Okamura H, Hanaoka F, Ohkuma Y, Nishimura Y, EMBO J. 2000 Mar 15;19(6):1346-56. PMID:10716934
Page seeded by OCA on Sun Mar 30 19:36:45 2008
