1ddp
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1ddp |SIZE=350|CAPTION= <scene name='initialview01'>1ddp</scene> | |PDB= 1ddp |SIZE=350|CAPTION= <scene name='initialview01'>1ddp</scene> | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CPT:CIS-PLATINUM-(NH3)2'>CPT</scene> | + | |LIGAND= <scene name='pdbligand=CPT:CIS-PLATINUM-(NH3)2'>CPT</scene>, <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ddp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ddp OCA], [http://www.ebi.ac.uk/pdbsum/1ddp PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ddp RCSB]</span> | ||
}} | }} | ||
| Line 26: | Line 29: | ||
[[Category: Reid, B R.]] | [[Category: Reid, B R.]] | ||
[[Category: Zhu, L.]] | [[Category: Zhu, L.]] | ||
| - | [[Category: CPT]] | ||
[[Category: antitumor drug]] | [[Category: antitumor drug]] | ||
[[Category: cis-ddp]] | [[Category: cis-ddp]] | ||
| Line 34: | Line 36: | ||
[[Category: nmr]] | [[Category: nmr]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:39:35 2008'' |
Revision as of 16:39, 30 March 2008
| |||||||
| Ligands: | , , , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
SOLUTION STRUCTURE OF A CISPLATIN-INDUCED [CATAGCTATG]2 INTERSTRAND CROSS-LINK
Overview
The widely used antitumor drug cis-diamminedichloroplatinum(II) (cisplatin or cis-DDP) reacts with DNA, cross-linking two purine residues through the N7 atoms, which reside in the major groove in B-form DNA. The solution structure of the short duplex [d(CAT-AGCTATG)]2 cross-linked at the GC:GC site was determined by nuclear magnetic resonance (NMR). The deoxyguanosine-bridging cis-diammineplatinum(II) lies in the minor groove, and the complementary deoxycytidines are extrahelical. The double helix is locally reversed to a left-handed form, and the helix is unwound and bent toward the minor groove. These findings were independently confirmed by results from a phase-sensitive gel electrophoresis bending assay. The NMR structure differs markedly from previously proposed models but accounts for the chemical reactivity, the unwinding, and the bending of cis-DDP interstrand cross-linked DNA and may be important in the formation and repair of these cross-links in chromatin.
About this Structure
1DDP is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Solution structure of a cisplatin-induced DNA interstrand cross-link., Huang H, Zhu L, Reid BR, Drobny GP, Hopkins PB, Science. 1995 Dec 15;270(5243):1842-5. PMID:8525382
Page seeded by OCA on Sun Mar 30 19:39:35 2008
Categories: Protein complex | Drobny, G P. | Hopkins, P B. | Huang, H. | Reid, B R. | Zhu, L. | Antitumor drug | Cis-ddp | Dna | Double helix | Interstrand cross-link | Nmr
