1dum

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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dum FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dum OCA], [http://www.ebi.ac.uk/pdbsum/1dum PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1dum RCSB]</span>
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[[Category: vesicle]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:49:09 2008''

Revision as of 16:49, 30 March 2008

Image:1dum.gif


PDB ID 1dum

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Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



NMR STRUCTURE OF [F5Y, F16W] MAGAININ 2 BOUND TO PHOSPHOLIPID VESICLES


Overview

To elucidate the effects of peptide dimerization on pore formation by magainin 2 (MG2), a covalently linked antiparallel dimer of the MG2 analogue [(F5Y, L6C, F16W, I20C-MG2)(2): II] was synthesized based on the dimer structure revealed by our NMR study. The interactions of the dimer with lipid bilayers were investigated by CD and fluorescence in comparison with a monomer analogue (F5Y, F16W-MG2: I). Similar to I, II was found to form a peptide-lipid supramolecular complex pore accompanied with lipid flip-flop and peptide translocation. The pore formed by II was characterized by a slightly larger pore diameter and a threefold longer lifetime than that of I, although the pore formation rate of the dimer was lower than that of the monomer. The coexistence of the dimer and the monomer exhibited slight but significant synergism in membrane permeabilization, which was maximal at a monomer/dimer ratio of 3. Therefore, we concluded that a pentameric pore composed of one pore-stabilizing dimer and three monomers maximized the overall leakage activity in keeping with our kinetic prediction.

About this Structure

1DUM is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.

Reference

Effects of peptide dimerization on pore formation: Antiparallel disulfide-dimerized magainin 2 analogue., Hara T, Kodama H, Kondo M, Wakamatsu K, Takeda A, Tachi T, Matsuzaki K, Biopolymers. 2001 Apr 5;58(4):437-46. PMID:11180056

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