1dw4
From Proteopedia
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|PDB= 1dw4 |SIZE=350|CAPTION= <scene name='initialview01'>1dw4</scene> | |PDB= 1dw4 |SIZE=350|CAPTION= <scene name='initialview01'>1dw4</scene> | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=NH2:AMINO GROUP'>NH2</scene> | + | |LIGAND= <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1dw5|1DW5]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dw4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dw4 OCA], [http://www.ebi.ac.uk/pdbsum/1dw4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1dw4 RCSB]</span> | ||
}} | }} | ||
| Line 26: | Line 29: | ||
[[Category: Lefevre, J F.]] | [[Category: Lefevre, J F.]] | ||
[[Category: Sirockin, F.]] | [[Category: Sirockin, F.]] | ||
| - | [[Category: NH2]] | ||
[[Category: calcium channel]] | [[Category: calcium channel]] | ||
[[Category: conformational exchange]] | [[Category: conformational exchange]] | ||
[[Category: conotoxin]] | [[Category: conotoxin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:49:57 2008'' |
Revision as of 16:50, 30 March 2008
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| Ligands: | |||||||
| Related: | 1DW5
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
NMR STRUCTURE OF OMEGA-CONOTOXIN MVIIA: CONSTRAINTS ON DISULPHIDE BRIDGES
Overview
omega-Conotoxin MVIIA is a 25-residue, disulfide-bridged polypeptide from the venom of the sea snail Conus magus that binds to neuronal N-type calcium channels. It forms a compact folded structure, presenting a loop between Cys8 and Cys15 that contains a set of residues critical for its binding. The loop does not have a unique defined structure, nor is it intrinsically flexible. Broadening of a subset of resonances in the NMR spectrum at low temperature, anomalous temperature dependence of the chemical shifts of some resonances, and exchange contributions to J(0) from (13)C relaxation measurements reveal that conformational exchange affects the residues in this loop. The effects of this exchange on the calculated structure of omega-conotoxin MVIIA are discussed. The exchange appears to be associated with a change in the conformation of the disulfide bridge Cys8-Cys20. The implications for the use of the omega-conotoxins as a scaffold for carrying other functions is discussed.
About this Structure
1DW4 is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
Structural and dynamic characterization of omega-conotoxin MVIIA: the binding loop exhibits slow conformational exchange., Atkinson RA, Kieffer B, Dejaegere A, Sirockin F, Lefevre JF, Biochemistry. 2000 Apr 11;39(14):3908-19. PMID:10747778
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