Structural highlights
Function
[GRAU_DROME] Transcription factor essential for the completion of meiosis in oocytes. Grauzone binds to the promoter region of cort via the zf-AD domain and activates cort expression in ovaries.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
About one-third of the more than 300 C2H2 zinc finger proteins of Drosophila contain a conserved sequence motif, the zinc finger-associated domain (ZAD). Genes that encode ZAD proteins are specific for and expanded in the genomes of insects. Only three ZAD-encoding gene functions are established, and the role of ZAD is unknown. Here we present the crystal structure of the ZAD of Grauzone (ZAD(Grau)), a Drosophila transcription factor that specifically controls the maternal Cdc20-like APC subunit Cortex. ZAD forms an atypical treble-clef-like zinc-coordinating fold. Head-to-tail arrangement of two ZAD(Grau) molecules in the crystals suggests dimer formation, an observation supported by crosslinking and dynamic light scattering. The results indicate that ZAD provides a novel protein-protein interaction module that characterizes a large family of insect transcription factors.
The zinc finger-associated domain of the Drosophila transcription factor grauzone is a novel zinc-coordinating protein-protein interaction module.,Jauch R, Bourenkov GP, Chung HR, Urlaub H, Reidt U, Jackle H, Wahl MC Structure. 2003 Nov;11(11):1393-402. PMID:14604529[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Chen B, Harms E, Chu T, Henrion G, Strickland S. Completion of meiosis in Drosophila oocytes requires transcriptional control by grauzone, a new zinc finger protein. Development. 2000 Mar;127(6):1243-51. PMID:10683177
- ↑ Harms E, Chu T, Henrion G, Strickland S. The only function of Grauzone required for Drosophila oocyte meiosis is transcriptional activation of the cortex gene. Genetics. 2000 Aug;155(4):1831-9. PMID:10924478
- ↑ Jauch R, Bourenkov GP, Chung HR, Urlaub H, Reidt U, Jackle H, Wahl MC. The zinc finger-associated domain of the Drosophila transcription factor grauzone is a novel zinc-coordinating protein-protein interaction module. Structure. 2003 Nov;11(11):1393-402. PMID:14604529
