Structural highlights
2e6h is a 4 chain structure with sequence from Thet8. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Ligands: | , , |
| Related: | 2e69, 2e6b, 2e6c, 2e6e, 2e6g, 1j9j, 1ilv, 1j9l, 1j9k, 1l5x |
| Activity: | 5'-nucleotidase, with EC number 3.1.3.5 |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum |
Function
[SURE_THET8] Nucleotidase that shows phosphatase activity on nucleoside 5'-monophosphates (By similarity).
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The stationary phase survival protein SurE is a metal ion-dependent phosphatase distributed among eubacteria, archaea, and eukaryotes. In Escherichia coli, SurE has activities as nucleotidase and exopolyphosphatase, and is thought to be involved in stress response. However, its physiological role and reaction mechanism are unclear. We report here the crystal structures of the tetramer of SurE from Thermus thermophilus HB8 (TtSurE) both alone and crystallized with Mn(2+) and substrate AMP. In the presence of Mn(2+) and AMP, differences between the protomers were observed in the active site and in the loop located near the active site; AMP-bound active sites with the loops in a novel open conformation were found in the two protomers, and AMP-free active sites with the loops in a conventional closed conformation were found in the other two protomers. The two loops in the open conformation are entwined with each other, and this entwining is suggested to be required for enzymatic activity by site-directed mutagenesis. TtSurE exists as an equilibrium mixture of dimer and tetramer in solution. The loop-entwined structure indicates that SurE acts as a tetramer. The structural features and the absence of negative cooperativity imply the half-of-the-sites reactivity mechanism resulting from a pre-existing tendency toward structural asymmetry.
Crystal structure of the stationary phase survival protein SurE with metal ion and AMP.,Iwasaki W, Miki K J Mol Biol. 2007 Aug 3;371(1):123-36. Epub 2007 May 10. PMID:17561111[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Iwasaki W, Miki K. Crystal structure of the stationary phase survival protein SurE with metal ion and AMP. J Mol Biol. 2007 Aug 3;371(1):123-36. Epub 2007 May 10. PMID:17561111 doi:10.1016/j.jmb.2007.05.007
