1ebu
From Proteopedia
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|PDB= 1ebu |SIZE=350|CAPTION= <scene name='initialview01'>1ebu</scene>, resolution 2.60Å | |PDB= 1ebu |SIZE=350|CAPTION= <scene name='initialview01'>1ebu</scene>, resolution 2.60Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NDA:3-AMINOMETHYL-PYRIDINIUM-ADENINE-DINUCLEOTIDE'>NDA</scene> | + | |LIGAND= <scene name='pdbligand=HSE:L-HOMOSERINE'>HSE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NDA:3-AMINOMETHYL-PYRIDINIUM-ADENINE-DINUCLEOTIDE'>NDA</scene> |
| - | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Homoserine_dehydrogenase Homoserine dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.3 1.1.1.3] </span> | |
|GENE= HOM6P ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | |GENE= HOM6P ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1ebf|1EBF]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ebu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ebu OCA], [http://www.ebi.ac.uk/pdbsum/1ebu PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ebu RCSB]</span> | ||
}} | }} | ||
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[[Category: Thompson, P R.]] | [[Category: Thompson, P R.]] | ||
[[Category: Wright, G D.]] | [[Category: Wright, G D.]] | ||
| - | [[Category: HSE]] | ||
| - | [[Category: NA]] | ||
| - | [[Category: NDA]] | ||
[[Category: analogue]] | [[Category: analogue]] | ||
[[Category: dehydrogenase]] | [[Category: dehydrogenase]] | ||
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[[Category: ternary]] | [[Category: ternary]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:59:25 2008'' |
Revision as of 16:59, 30 March 2008
| |||||||
| , resolution 2.60Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Gene: | HOM6P (Saccharomyces cerevisiae) | ||||||
| Activity: | Homoserine dehydrogenase, with EC number 1.1.1.3 | ||||||
| Related: | 1EBF
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
HOMOSERINE DEHYDROGENASE COMPLEX WITH NAD ANALOGUE AND L-HOMOSERINE
Overview
The structure of the antifungal drug target homoserine dehydrogenase (HSD) was determined from Saccharomyces cerevisiae in apo and holo forms, and as a ternary complex with bound products, by X-ray diffraction. The three forms show that the enzyme is a dimer, with each monomer composed of three regions, the nucleotide-binding region, the dimerization region and the catalytic region. The dimerization and catalytic regions have novel folds, whereas the fold of the nucleotide-binding region is a variation on the Rossmann fold. The novel folds impose a novel composition and arrangement of active site residues when compared to all other currently known oxidoreductases. This observation, in conjunction with site-directed mutagenesis of active site residues and steady-state kinetic measurements, suggest that HSD exhibits a new variation on dehydrogenase chemistry.
About this Structure
1EBU is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Crystal structures of homoserine dehydrogenase suggest a novel catalytic mechanism for oxidoreductases., DeLaBarre B, Thompson PR, Wright GD, Berghuis AM, Nat Struct Biol. 2000 Mar;7(3):238-44. PMID:10700284
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