1edm
From Proteopedia
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|PDB= 1edm |SIZE=350|CAPTION= <scene name='initialview01'>1edm</scene>, resolution 1.5Å | |PDB= 1edm |SIZE=350|CAPTION= <scene name='initialview01'>1edm</scene>, resolution 1.5Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= HUMAN FACTOR IX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |GENE= HUMAN FACTOR IX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1edm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1edm OCA], [http://www.ebi.ac.uk/pdbsum/1edm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1edm RCSB]</span> | ||
}} | }} | ||
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==Overview== | ==Overview== | ||
Various diverse extracellular proteins possess Ca(2+)-binding epidermal growth factor (EGF)-like domains, the function of which remains uncertain. We have determined, at high resolution (1.5 A), the crystal structure of such a domain, from human clotting factor IX, as a complex with Ca2+. The Ca2+ ligands form a classic pentagonal bipyramid with six ligands contributed by one polypeptide chain and the seventh supplied by a neighboring EGF-like domain. The crystal structure identifies the role of Ca2+ in maintaining the conformation of the N-terminal region of the domain, but more importantly demonstrates that Ca2+ can directly mediate protein-protein contacts. The observed crystal packing of the domains provides a plausible model for the association of multiple tandemly linked EGF-like domains in proteins such as fibrillin-1, Notch, and protein S. This model is consistent with the known functional data and suggests a general biological role for these domains. | Various diverse extracellular proteins possess Ca(2+)-binding epidermal growth factor (EGF)-like domains, the function of which remains uncertain. We have determined, at high resolution (1.5 A), the crystal structure of such a domain, from human clotting factor IX, as a complex with Ca2+. The Ca2+ ligands form a classic pentagonal bipyramid with six ligands contributed by one polypeptide chain and the seventh supplied by a neighboring EGF-like domain. The crystal structure identifies the role of Ca2+ in maintaining the conformation of the N-terminal region of the domain, but more importantly demonstrates that Ca2+ can directly mediate protein-protein contacts. The observed crystal packing of the domains provides a plausible model for the association of multiple tandemly linked EGF-like domains in proteins such as fibrillin-1, Notch, and protein S. This model is consistent with the known functional data and suggests a general biological role for these domains. | ||
| - | |||
| - | ==Disease== | ||
| - | Known diseases associated with this structure: Hemophilia B OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=306900 306900]], Warfarin sensitivity OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=306900 306900]] | ||
==About this Structure== | ==About this Structure== | ||
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[[Category: Rao, Z.]] | [[Category: Rao, Z.]] | ||
[[Category: Stuart, D.]] | [[Category: Stuart, D.]] | ||
| - | [[Category: CA]] | ||
[[Category: calcium-binding]] | [[Category: calcium-binding]] | ||
[[Category: coagulation factor]] | [[Category: coagulation factor]] | ||
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[[Category: structure and function]] | [[Category: structure and function]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:00:32 2008'' |
Revision as of 17:00, 30 March 2008
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| , resolution 1.5Å | |||||||
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| Ligands: | |||||||
| Gene: | HUMAN FACTOR IX (Homo sapiens) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
EPIDERMAL GROWTH FACTOR-LIKE DOMAIN FROM HUMAN FACTOR IX
Overview
Various diverse extracellular proteins possess Ca(2+)-binding epidermal growth factor (EGF)-like domains, the function of which remains uncertain. We have determined, at high resolution (1.5 A), the crystal structure of such a domain, from human clotting factor IX, as a complex with Ca2+. The Ca2+ ligands form a classic pentagonal bipyramid with six ligands contributed by one polypeptide chain and the seventh supplied by a neighboring EGF-like domain. The crystal structure identifies the role of Ca2+ in maintaining the conformation of the N-terminal region of the domain, but more importantly demonstrates that Ca2+ can directly mediate protein-protein contacts. The observed crystal packing of the domains provides a plausible model for the association of multiple tandemly linked EGF-like domains in proteins such as fibrillin-1, Notch, and protein S. This model is consistent with the known functional data and suggests a general biological role for these domains.
About this Structure
1EDM is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The structure of a Ca(2+)-binding epidermal growth factor-like domain: its role in protein-protein interactions., Rao Z, Handford P, Mayhew M, Knott V, Brownlee GG, Stuart D, Cell. 1995 Jul 14;82(1):131-41. PMID:7606779
Page seeded by OCA on Sun Mar 30 20:00:32 2008
