1efa
From Proteopedia
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|PDB= 1efa |SIZE=350|CAPTION= <scene name='initialview01'>1efa</scene>, resolution 2.60Å | |PDB= 1efa |SIZE=350|CAPTION= <scene name='initialview01'>1efa</scene>, resolution 2.60Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=NPF:ORTHONITROPHENYL-BETA-D-FUCOPYRANOSIDE'>NPF</scene> | + | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=NPF:ORTHONITROPHENYL-BETA-D-FUCOPYRANOSIDE'>NPF</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1efa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1efa OCA], [http://www.ebi.ac.uk/pdbsum/1efa PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1efa RCSB]</span> | ||
}} | }} | ||
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[[Category: Bell, C E.]] | [[Category: Bell, C E.]] | ||
[[Category: Lewis, M.]] | [[Category: Lewis, M.]] | ||
| - | [[Category: NPF]] | ||
[[Category: gene regulation]] | [[Category: gene regulation]] | ||
[[Category: helix-turn-helix]] | [[Category: helix-turn-helix]] | ||
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[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:01:21 2008'' |
Revision as of 17:01, 30 March 2008
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| , resolution 2.60Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF THE LAC REPRESSOR DIMER BOUND TO OPERATOR AND THE ANTI-INDUCER ONPF
Overview
Crystal structures of the Lac repressor, with and without isopropyithiogalactoside (IPTG), and the repressor bound to operator have provided a model for how the binding of the inducer reduces the affinity of the repressor for the operator. However, because of the low resolution of the operator-bound structure (4.8 A), the model for the allosteric transition was presented in terms of structural elements rather than in terms of side chain interactions. Here we have constructed a dimeric Lac repressor and determined its structure at 2.6 A resolution in complex with a symmetric operator and the anti-inducer orthonitrophenylfucoside (ONPF). The structure enables the induced (IPTG-bound) and repressed (operator-bound) conformations of the repressor to be compared in atomic detail. An extensive network of interactions between the DNA-binding and core domains of the repressor suggests a possible mechanism for the allosteric transition.
About this Structure
1EFA is a Single protein structure of sequence from Escherichia coli. The following page contains interesting information on the relation of 1EFA with [lac Repressor]. Full crystallographic information is available from OCA.
Reference
A closer view of the conformation of the Lac repressor bound to operator., Bell CE, Lewis M, Nat Struct Biol. 2000 Mar;7(3):209-14. PMID:10700279
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