This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1efa
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1efa |SIZE=350|CAPTION= <scene name='initialview01'>1efa</scene>, resolution 2.60Å | |PDB= 1efa |SIZE=350|CAPTION= <scene name='initialview01'>1efa</scene>, resolution 2.60Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=NPF:ORTHONITROPHENYL-BETA-D-FUCOPYRANOSIDE'>NPF</scene> | + | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=NPF:ORTHONITROPHENYL-BETA-D-FUCOPYRANOSIDE'>NPF</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1efa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1efa OCA], [http://www.ebi.ac.uk/pdbsum/1efa PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1efa RCSB]</span> | ||
}} | }} | ||
| Line 25: | Line 28: | ||
[[Category: Bell, C E.]] | [[Category: Bell, C E.]] | ||
[[Category: Lewis, M.]] | [[Category: Lewis, M.]] | ||
| - | [[Category: NPF]] | ||
[[Category: gene regulation]] | [[Category: gene regulation]] | ||
[[Category: helix-turn-helix]] | [[Category: helix-turn-helix]] | ||
| Line 31: | Line 33: | ||
[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:01:21 2008'' |
Revision as of 17:01, 30 March 2008
| |||||||
| , resolution 2.60Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF THE LAC REPRESSOR DIMER BOUND TO OPERATOR AND THE ANTI-INDUCER ONPF
Overview
Crystal structures of the Lac repressor, with and without isopropyithiogalactoside (IPTG), and the repressor bound to operator have provided a model for how the binding of the inducer reduces the affinity of the repressor for the operator. However, because of the low resolution of the operator-bound structure (4.8 A), the model for the allosteric transition was presented in terms of structural elements rather than in terms of side chain interactions. Here we have constructed a dimeric Lac repressor and determined its structure at 2.6 A resolution in complex with a symmetric operator and the anti-inducer orthonitrophenylfucoside (ONPF). The structure enables the induced (IPTG-bound) and repressed (operator-bound) conformations of the repressor to be compared in atomic detail. An extensive network of interactions between the DNA-binding and core domains of the repressor suggests a possible mechanism for the allosteric transition.
About this Structure
1EFA is a Single protein structure of sequence from Escherichia coli. The following page contains interesting information on the relation of 1EFA with [lac Repressor]. Full crystallographic information is available from OCA.
Reference
A closer view of the conformation of the Lac repressor bound to operator., Bell CE, Lewis M, Nat Struct Biol. 2000 Mar;7(3):209-14. PMID:10700279
Page seeded by OCA on Sun Mar 30 20:01:21 2008
