Enzymes: enzyme-substrate complex

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(New page: es:Enzymes: enzyme-substrate complex (spanish) <Structure load='4ald' size='800' frame='true' align='right' caption='Fructosa-bifosfato aldolasa (PDB 4ald)' scene='60/603296/F2pa/4...)
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:::<big><big>Utilizando modelos moleculares 3D vamos a realizar una aproximación a la relación espacial entre los enzimas y sus respectivos sustratos. Utilizaremos como ejemplo el enzima ''fructosa-bifosfato aldolasa'', un enzima de la secuencia glucolítica que cataliza la escisión de la ''fructosa-1,6-bifosfato'' (su sustrato) para dar lugar a una molécula de ''gliceraldehido-3-fosfato'' y otra de ''dihidroxiacetona-fosfato''. En la ventana de la derecha podemos apreciar, por medio de un <scene name='60/603296/F2pa/4'>modelo de cintas</scene>, las estructuras secundarias predominantes en la molécula de la ''fructosa-bifosfato aldolasa''.
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:::<big><big>
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:::En el modelo de <scene name='60/603296/F2pa/3'>malla de alambre</scene>, en el que se representa tanto el esqueleto de la cadena polipeptídica como las cadenas laterales de los residuos de aminoácidos, se aprecia la gran complejidad de esta molécula enzimática formada por <scene name='60/603296/F2pa/17'>363 aminoácidos</scene>.
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:::*Enzyme: '''Fructose biphosphate aldolase'''
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:::Procedamos ahora a <scene name='60/603296/F2pa/5'>atenuar</scene> el conjunto de la cadena polipeptídica para centrar nuestra atención en el <scene name='60/603296/F2pa/6'>centro activo</scene> del enzima, una cavidad rodeada de <scene name='60/603296/F2pa/7'>9 residuos</scene> en la que encaja el <scene name='60/603296/F2pa/9'>sustrato</scene>, en este caso la molécula de ''fructosa-1,6-bifosfato''.
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:::*Substrate: '''fructose-1,6-biphosphate'''
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:::Con el objeto de visualizar mejor el <scene name='60/603296/F2pa/10'>acoplamiento espacial</scene> entre e centro activo y la molécula de sustrato se representan ahora los aminoácidos del centro activo y la molécula de sustrato con sus átomos ampliados hasta su radio de Van der Waals. A continuación, nos alejamos para poder apreciar una vista general del <scene name='60/603296/F2pa/11'>complejo enzima-sustrato</scene>.
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:::*Products: '''glyceraldehyde-3-phosphate''' and '''dihydroxiacetone-phosphate''.
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:::This <scene name='60/603296/F2pa/4'>ribbon model</scene> shows secondariy structures in '''Fructose biphosphate aldolase''' molecule.
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:::This <scene name='60/603296/F2pa/3'>wireframe model</scene> shows the polypeptide chain skeleton and R groups.
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:::The polypeptide chain of this enzyme consist of <scene name='60/603296/F2pa/17'>363 amino acid resiues</scene>.
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:::Now, we are going to <scene name='60/603296/F2pa/5'>soften colors</scene> to highligth enzyme centrar nuestra atención en el <scene name='60/603296/F2pa/6'>active site</scene>
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:::Active site is surrounded by <scene name='60/603296/F2pa/7'>9 amino acid residues</scene>
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:::<scene name='60/603296/F2pa/9'>Substrate molecule</scene>, i.e., '''fructose-1,6-biphosphate'''. fits into active site.
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:::In this <scene name='60/603296/F2pa/10'>spacefill model</scene>, fit between active site and substrate is best seen.
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:::Zoom out to see a <scene name='60/603296/F2pa/11'>enzyme-substrate complex</scene> whole view.
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::Todos los modelos que hemos analizado corresponden a una única <scene name='60/603296/F2pa/15'>subunidad</scene> de la ''fructosa-bifosfato aldolasa'', que es en realidad una proteína oligomérica formada por cuatro subunidades que conforman un <scene name='60/603296/F2pa/16'>tetrámero</scene>.
 
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== Atribución de contenidos ==
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::All models we have analyzed show a <scene name='60/603296/F2pa/15'>single subunit</scene> of '''fructose biphosphate aldolase'''. Really, this enzyme is a oligomeric protein composed of four subunits that perform a <scene name='60/603296/F2pa/16'>tetramer</scene>.
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Esta página está basada en el archivo [[4ald]] de la Proteopedia.
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==References==
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This page is based on the Proteopedia file [[4ald]].
<references/>
<references/>

Revision as of 11:09, 9 February 2016

Fructosa-bifosfato aldolasa (PDB 4ald)

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  • Enzyme: Fructose biphosphate aldolase
  • Substrate: fructose-1,6-biphosphate
  • Products: glyceraldehyde-3-phosphate' and dihydroxiacetone-phosphate.
This shows secondariy structures in Fructose biphosphate aldolase molecule.
This shows the polypeptide chain skeleton and R groups.
The polypeptide chain of this enzyme consist of .
Now, we are going to to highligth enzyme centrar nuestra atención en el
Active site is surrounded by
, i.e., fructose-1,6-biphosphate. fits into active site.
In this , fit between active site and substrate is best seen.
Zoom out to see a whole view.


All models we have analyzed show a of fructose biphosphate aldolase. Really, this enzyme is a oligomeric protein composed of four subunits that perform a .

References

This page is based on the Proteopedia file 4ald.

Proteopedia Page Contributors and Editors (what is this?)

Alejandro Porto

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