1f1z
From Proteopedia
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|PDB= 1f1z |SIZE=350|CAPTION= <scene name='initialview01'>1f1z</scene>, resolution 2.40Å | |PDB= 1f1z |SIZE=350|CAPTION= <scene name='initialview01'>1f1z</scene>, resolution 2.40Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f1z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f1z OCA], [http://www.ebi.ac.uk/pdbsum/1f1z PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1f1z RCSB]</span> | ||
}} | }} | ||
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[[Category: Mathew, S V.]] | [[Category: Mathew, S V.]] | ||
[[Category: May, E W.]] | [[Category: May, E W.]] | ||
| - | [[Category: CL]] | ||
| - | [[Category: MG]] | ||
[[Category: restriction endonuclease fold]] | [[Category: restriction endonuclease fold]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:14:06 2008'' |
Revision as of 17:14, 30 March 2008
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| , resolution 2.40Å | |||||||
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| Ligands: | , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
TNSA, a catalytic component of the TN7 transposition system
Overview
Transposition requires a coordinated series of DNA breakage and joining reactions. The Tn7 transposase contains two proteins: TnsA, which carries out DNA breakage at the 5' ends of the transposon, and TnsB, which carries out breakage and joining at the 3' ends of the transposon. TnsB is a member of the retroviral integrase superfamily whose hallmark is a conserved DDE motif. We report here the structure of TnsA at 2.4 A resolution. Surprisingly, the TnsA fold is that of a type II restriction endonuclease. Thus, Tn7 transposition involves a collaboration between polypeptides, one containing a DDE motif and one that does not. This result indicates that the range of biological processes that utilize restriction enzyme-like folds also includes DNA transposition.
About this Structure
1F1Z is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Unexpected structural diversity in DNA recombination: the restriction endonuclease connection., Hickman AB, Li Y, Mathew SV, May EW, Craig NL, Dyda F, Mol Cell. 2000 Jun;5(6):1025-34. PMID:10911996
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