1f3s
From Proteopedia
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|PDB= 1f3s |SIZE=350|CAPTION= <scene name='initialview01'>1f3s</scene> | |PDB= 1f3s |SIZE=350|CAPTION= <scene name='initialview01'>1f3s</scene> | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f3s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f3s OCA], [http://www.ebi.ac.uk/pdbsum/1f3s PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1f3s RCSB]</span> | ||
}} | }} | ||
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[[Category: Patel, D J.]] | [[Category: Patel, D J.]] | ||
[[Category: Skripkin, E.]] | [[Category: Skripkin, E.]] | ||
| - | [[Category: 15n-labeled dna]] | ||
[[Category: g-quadruplex]] | [[Category: g-quadruplex]] | ||
[[Category: g.(c-a) base triad]] | [[Category: g.(c-a) base triad]] | ||
[[Category: triad dna model]] | [[Category: triad dna model]] | ||
| - | [[Category: uniform 13c]] | + | [[Category: uniform 13c,15n-labeled dna]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:15:12 2008'' |
Revision as of 17:15, 30 March 2008
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| Ligands: | , , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
SOLUTION STRUCTURE OF DNA SEQUENCE GGGTTCAGG FORMS GGGG TETRADE AND G(C-A) TRIAD.
Overview
We have designed a DNA sequence, d(G-G-G-T-T-C-A-G-G), which dimerizes to form a 2-fold symmetric G-quadruplex in which G(syn). G(anti).G(syn).G(anti) tetrads are sandwiched between all trans G. (C-A) triads. The NMR-based solution structural analysis was greatly aided by monitoring hydrogen bond alignments across N-H...N and N-H...O==C hydrogen bonds within the triad and tetrad, in a uniformly ((13)C,(15)N)-labeled sample of the d(G-G-G-T-T-C-A-G-G) sequence. The solution structure establishes that the guanine base-pairs with the cytosine through Watson-Crick G.C pair formation and with adenine through sheared G.A mismatch formation within the G.(C-A) triad. A model of triad DNA was constructed that contains the experimentally determined G.(C-A) triad alignment as the repeating stacked unit.
About this Structure
1F3S is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
A two-stranded template-based approach to G.(C-A) triad formation: designing novel structural elements into an existing DNA framework., Kettani A, Basu G, Gorin A, Majumdar A, Skripkin E, Patel DJ, J Mol Biol. 2000 Aug 4;301(1):129-46. PMID:10926497
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