1fhy
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1fhy |SIZE=350|CAPTION= <scene name='initialview01'>1fhy</scene>, resolution 2.20Å | |PDB= 1fhy |SIZE=350|CAPTION= <scene name='initialview01'>1fhy</scene>, resolution 2.20Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> | + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=PSO:4'-HYDROXYMETHYL-4,5',8-TRIMETHYLPSORALEN'>PSO</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fhy OCA], [http://www.ebi.ac.uk/pdbsum/1fhy PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1fhy RCSB]</span> | ||
}} | }} | ||
| Line 26: | Line 29: | ||
[[Category: Ho, P S.]] | [[Category: Ho, P S.]] | ||
[[Category: Mooers, B H.M.]] | [[Category: Mooers, B H.M.]] | ||
| - | [[Category: CA]] | ||
| - | [[Category: PSO]] | ||
[[Category: 4-way junction]] | [[Category: 4-way junction]] | ||
[[Category: cross-linked dna]] | [[Category: cross-linked dna]] | ||
| Line 34: | Line 35: | ||
[[Category: psoralen]] | [[Category: psoralen]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:23:15 2008'' |
Revision as of 17:23, 30 March 2008
| |||||||
| , resolution 2.20Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
PSORALEN CROSS-LINKED D(CCGCTAGCGG) FORMS HOLLIDAY JUNCTION
Overview
The single-crystal structures are presented for two DNA sequences with the thymine bases covalently cross-linked across the complementary strands by 4'-hydroxymethyl-4,5',8-trimethylpsoralen (HMT). The HMT-adduct of d(CCGCTAGCGG) forms a psoralen-induced Holliday junction, showing for the first time the effect of this important class of chemotheraputics on the structure of the recombination intermediate. In contrast, HMT-d(CCGGTACCGG) forms a sequence-dependent junction. In both structures, the DNA duplex is highly distorted at the thymine base linked to the six-member pyrone ring of the drug. The psoralen cross-link defines the intramolecular interactions of the drug-induced junction, while the sequence-dependent structure is nearly identical to the native Holliday junction of d(CCGGTACCGG) alone. The two structures contrast the effects of drug- and sequence-dependent interactions on the structure of a Holliday junction, suggesting a role for psoralen in the mechanism to initiate repair of psoralen-lesions in mammalian DNA.
About this Structure
1FHY is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
The crystal structures of psoralen cross-linked DNAs: drug-dependent formation of Holliday junctions., Eichman BF, Mooers BH, Alberti M, Hearst JE, Ho PS, J Mol Biol. 2001 Apr 20;308(1):15-26. PMID:11302703
Page seeded by OCA on Sun Mar 30 20:23:15 2008
