1fl8

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|PDB= 1fl8 |SIZE=350|CAPTION= <scene name='initialview01'>1fl8</scene>
|PDB= 1fl8 |SIZE=350|CAPTION= <scene name='initialview01'>1fl8</scene>
|SITE=
|SITE=
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|LIGAND=
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|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=PSU:PSEUDOURIDINE-5&#39;-MONOPHOSPHATE'>PSU</scene>, <scene name='pdbligand=T6A:N-[N-(9-B-D-RIBOFURANOSYLPURIN-6-YL)CARBAMOYL]THREONINE-5&#39;-MONOPHOSPHATE'>T6A</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>, <scene name='pdbligand=U8U:5-METHYLAMINOMETHYL-2-THIOURIDINE-5&#39;-MONOPHOSPHATE'>U8U</scene>
|ACTIVITY=
|ACTIVITY=
|GENE=
|GENE=
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|DOMAIN=
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|RELATEDENTRY=[[1bzu|1BZU]], [[1bz2|1BZ2]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fl8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fl8 OCA], [http://www.ebi.ac.uk/pdbsum/1fl8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1fl8 RCSB]</span>
}}
}}
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[[Category: trna]]
[[Category: trna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:10:32 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:25:07 2008''

Revision as of 17:25, 30 March 2008

Image:1fl8.gif


PDB ID 1fl8

Drag the structure with the mouse to rotate
Ligands: , , , , , ,
Related: 1BZU, 1BZ2


Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



HYPERMODIFIED NUCLEOSIDES IN THE ANTICODON OF TRNALYS STABILIZE A CANONICAL U-TURN STRUCTURE


Overview

Modified nucleosides in the anticodon domain of Escherichia coli tRNA(Lys) are necessary for high-affinity codon recognition and reading frame maintenance. Human tRNA(Lys,3) is the specific primer for HIV-1 reverse transcriptase and also requires nucleoside modification for proper function. We now present NMR solution structures for the fully modified 17-nucleotide E. coli tRNA(Lys) anticodon stem-loop domain (ASL). NMR data were also collected for several partially modified ASLs, revealing the contributions each modified nucleoside (mnm(5)s(2)U34, t(6)A37, and psi39) makes in transforming the disordered, unmodified tRNA ASL into the highly ordered native structure. The solution structure of the native ASL domain provides insight into longstanding questions regarding both wobble position modification and the nearly ubiquitous t(6)A37 found in tRNAs with an adjacent U at position 36. Native tRNA(Lys) has a U-turn structure similar to the yeast tRNA(Phe) crystal structure, unlike previously proposed "unconventional" anticodon structures characterized by stable interactions between mnm(5)s(2)U-34 and t(6)A-37.

About this Structure

1FL8 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

Hypermodified nucleosides in the anticodon of tRNALys stabilize a canonical U-turn structure., Sundaram M, Durant PC, Davis DR, Biochemistry. 2000 Oct 17;39(41):12575-84. PMID:11027137

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