1fo8
From Proteopedia
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|PDB= 1fo8 |SIZE=350|CAPTION= <scene name='initialview01'>1fo8</scene>, resolution 1.40Å | |PDB= 1fo8 |SIZE=350|CAPTION= <scene name='initialview01'>1fo8</scene>, resolution 1.40Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=MMC:METHYL MERCURY ION'>MMC</scene> | + | |LIGAND= <scene name='pdbligand=MMC:METHYL+MERCURY+ION'>MMC</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Alpha-1,3-mannosyl-glycoprotein_2-beta-N-acetylglucosaminyltransferase Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.101 2.4.1.101] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-1,3-mannosyl-glycoprotein_2-beta-N-acetylglucosaminyltransferase Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.101 2.4.1.101] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1fo9|1FO9]], [[1foa|1FOA]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fo8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fo8 OCA], [http://www.ebi.ac.uk/pdbsum/1fo8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1fo8 RCSB]</span> | ||
}} | }} | ||
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[[Category: Yuwaraj, S.]] | [[Category: Yuwaraj, S.]] | ||
[[Category: Zhou, S.]] | [[Category: Zhou, S.]] | ||
| - | [[Category: | + | [[Category: alpha-1,3-mannosyl-glycoprotein beta-1,2-n-acetylglucosaminyltransferase]] |
| - | + | ||
| - | + | ||
| - | + | ||
[[Category: methylmercury derivative]] | [[Category: methylmercury derivative]] | ||
[[Category: n-acetylglucosaminyltransferase i]] | [[Category: n-acetylglucosaminyltransferase i]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:26:53 2008'' |
Revision as of 17:26, 30 March 2008
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| , resolution 1.40Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Activity: | Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, with EC number 2.4.1.101 | ||||||
| Related: | 1FO9, 1FOA
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF N-ACETYLGLUCOSAMINYLTRANSFERASE I
Overview
N:-acetylglucosaminyltransferase I (GnT I) serves as the gateway from oligomannose to hybrid and complex N:-glycans and plays a critical role in mammalian development and possibly all metazoans. We have determined the X-ray crystal structure of the catalytic fragment of GnT I in the absence and presence of bound UDP-GlcNAc/Mn(2+) at 1.5 and 1.8 A resolution, respectively. The structures identify residues critical for substrate binding and catalysis and provide evidence for similarity, at the mechanistic level, to the deglycosylation step of retaining beta-glycosidases. The structuring of a 13 residue loop, resulting from UDP-GlcNAc/Mn(2+) binding, provides an explanation for the ordered sequential 'Bi Bi' kinetics shown by GnT I. Analysis reveals a domain shared with Bacillus subtilis glycosyltransferase SpsA, bovine beta-1,4-galactosyl transferase 1 and Escherichia coli N:-acetylglucosamine-1-phosphate uridyltransferase. The low sequence identity, conserved fold and related functional features shown by this domain define a superfamily whose members probably share a common ancestor. Sequence analysis and protein threading show that the domain is represented in proteins from several glycosyltransferase families.
About this Structure
1FO8 is a Single protein structure of sequence from Oryctolagus cuniculus. Full crystallographic information is available from OCA.
Reference
X-ray crystal structure of rabbit N-acetylglucosaminyltransferase I: catalytic mechanism and a new protein superfamily., Unligil UM, Zhou S, Yuwaraj S, Sarkar M, Schachter H, Rini JM, EMBO J. 2000 Oct 16;19(20):5269-80. PMID:11032794
Page seeded by OCA on Sun Mar 30 20:26:53 2008
Categories: Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase | Oryctolagus cuniculus | Single protein | Rini, J M. | Sarkar, M. | Schachter, H. | Unligil, U M. | Yuwaraj, S. | Zhou, S. | Alpha-1,3-mannosyl-glycoprotein beta-1,2-n-acetylglucosaminyltransferase | Methylmercury derivative | N-acetylglucosaminyltransferase i
