1fre
From Proteopedia
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|PDB= 1fre |SIZE=350|CAPTION= <scene name='initialview01'>1fre</scene> | |PDB= 1fre |SIZE=350|CAPTION= <scene name='initialview01'>1fre</scene> | ||
|SITE= <scene name='pdbsite=ZN1:Zn+Binding+Site+1'>ZN1</scene> | |SITE= <scene name='pdbsite=ZN1:Zn+Binding+Site+1'>ZN1</scene> | ||
| - | |LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | + | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fre FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fre OCA], [http://www.ebi.ac.uk/pdbsum/1fre PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1fre RCSB]</span> | ||
}} | }} | ||
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[[Category: Borden, K L.B.]] | [[Category: Borden, K L.B.]] | ||
[[Category: Freemont, P S.]] | [[Category: Freemont, P S.]] | ||
| - | [[Category: ZN]] | ||
[[Category: bbox]] | [[Category: bbox]] | ||
[[Category: development]] | [[Category: development]] | ||
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[[Category: zinc-binding protein]] | [[Category: zinc-binding protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:28:49 2008'' |
Revision as of 17:28, 30 March 2008
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
XNF7 BBOX, DEVELOPMENTAL PROTEIN, PH 7.5, 30 C, WITH ZINC, NMR, 1 STRUCTURE
Overview
Xenopus nuclear factor XNF7, a maternally expressed protein, functions in patterning of the embryo. XNF7 contains a number of defined protein domains implicated in the regulation of some developmental processes. Among these is a tripartite motif comprising a zinc-binding RING finger and B-box domain next to a predicted alpha-helical coiled-coil domain. Interestingly, this motif is found in a variety of protein including several proto-oncoproteins. Here we describe the solution structure of the XNF7 B-box zinc-binding domain determined at physiological pH by 1H NMR methods. The B-box structure represents the first three-dimensional structure of this new motif and comprises a monomer have two beta-strands, two helical turns and three extended loop regions packed in a novel topology. The r.m.s. deviation for the best 18 structures is 1.15 A for backbone atoms and 1.94 A for all atoms. Structure calculations and biochemical data shows one zinc atom ligated in a Cys2-His2 tetrahedral arrangement. We have used mutant peptides to determine the metal ligation scheme which surprisingly shows that not all of the seven conserved cysteines/histidines in the B-box motif are involved in metal ligation. The B-box structure is not similar in tertiary fold to any other known zinc-binding motif.
About this Structure
1FRE is a Single protein structure of sequence from Xenopus laevis. Full crystallographic information is available from OCA.
Reference
Novel topology of a zinc-binding domain from a protein involved in regulating early Xenopus development., Borden KL, Lally JM, Martin SR, O'Reilly NJ, Etkin LD, Freemont PS, EMBO J. 1995 Dec 1;14(23):5947-56. PMID:8846787
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