4z5v
From Proteopedia
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| - | ''' | + | ==Crystal Structure of MHV ns2 PDE Domain== |
| + | <StructureSection load='4z5v' size='340' side='right' caption='[[4z5v]], [[Resolution|resolution]] 3.05Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4z5v]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Z5V FirstGlance]. <br> | ||
| + | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4z5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z5v OCA], [http://pdbe.org/4z5v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4z5v RCSB], [http://www.ebi.ac.uk/pdbsum/4z5v PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/NS2A_CVMA5 NS2A_CVMA5]] Not essential for virus replication in transformed murine cells. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Prior studies have demonstrated that the mouse hepatitis virus A59 strain (MHV-A59) ns2 protein is a member of the 2H phosphoesterase family and exhibits 2',5'-phosphodiesterase (2',5'-PDE) activity. During the interferon antiviral response, ns2 cleaves 2',5'-oligoadenylate (2-5A), a key mediator of RNase L activation, thereby subverting the activation of RNase L and evading host innate immunity. However, the mechanism of 2-5A cleavage by ns2 remains unclear. Here, we present the crystal structure of the MHV ns2 PDE domain and demonstrate a PDE fold similar to that of the cellular protein, a kinase anchoring protein 7 central domain (AKAP7CD) and rotavirus VP3 carboxy-terminal domain (VP3-CTD). The structure displays a pair of strictly conserved HxS/Tx motifs and forms a deep positively charged catalytic groove with beta-sheets and an arginine containing loop (R-loop). These findings provide insight into the structural basis for 2-5A binding of MHV ns2. | ||
| - | + | Crystal structure of the mouse hepatitis virus ns2 phosphodiesterase domain that antagonizes RNase L activation.,Sui B, Huang J, Jha BK, Yin P, Zhou M, Fu ZF, Silverman RH, Weiss SR, Peng G, Zhao L J Gen Virol. 2016 Jan 12. doi: 10.1099/jgv.0.000395. PMID:26757803<ref>PMID:26757803</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 4z5v" style="background-color:#fffaf0;"></div> | |
| - | [[Category: | + | == References == |
| - | [[Category: Peng, G | + | <references/> |
| - | [[Category: Sui, B | + | __TOC__ |
| - | + | </StructureSection> | |
| + | [[Category: Huang, J H]] | ||
| + | [[Category: Peng, G Q]] | ||
| + | [[Category: Sui, B K]] | ||
[[Category: Zhao, L]] | [[Category: Zhao, L]] | ||
| + | [[Category: Murine hepatitis virus]] | ||
| + | [[Category: Ns2 protein]] | ||
| + | [[Category: Phosphodiesterase]] | ||
| + | [[Category: Viral protein]] | ||
Revision as of 17:16, 10 February 2016
Crystal Structure of MHV ns2 PDE Domain
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