1fzp
From Proteopedia
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|PDB= 1fzp |SIZE=350|CAPTION= <scene name='initialview01'>1fzp</scene>, resolution 2.95Å | |PDB= 1fzp |SIZE=350|CAPTION= <scene name='initialview01'>1fzp</scene>, resolution 2.95Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1fzn|1FZN]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fzp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fzp OCA], [http://www.ebi.ac.uk/pdbsum/1fzp PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1fzp RCSB]</span> | ||
}} | }} | ||
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[[Category: Hurlburt, B.]] | [[Category: Hurlburt, B.]] | ||
[[Category: Schumacher, M A.]] | [[Category: Schumacher, M A.]] | ||
| - | [[Category: | + | [[Category: conformational changes,coil to helix]] |
| - | + | ||
| - | + | ||
[[Category: d-dna]] | [[Category: d-dna]] | ||
[[Category: indirect readout]] | [[Category: indirect readout]] | ||
[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:33:26 2008'' |
Revision as of 17:33, 30 March 2008
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| , resolution 2.95Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Related: | 1FZN
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURES OF SARA: A PLEIOTROPIC REGULATOR OF VIRULENCE GENES IN S. AUREUS
Overview
Staphylococcus aureus is a major human pathogen, the potency of which can be attributed to the regulated expression of an impressive array of virulence determinants. A key pleiotropic transcriptional regulator of these virulence factors is SarA, which is encoded by the sar (staphylococcal accessory regulator) locus. SarA was characterized initially as an activator of a second virulence regulatory locus, agr, through its interaction with a series of heptad repeats (AGTTAAG) within the agr promoter. Subsequent DNA-binding studies have revealed that SarA binds readily to multiple AT-rich sequences of variable lengths. Here we describe the crystal structure of SarA and a SarA-DNA complex at resolutions of 2.50 A and 2.95 A, respectively. SarA has a fold consisting of a four-helix core region and 'inducible regions' comprising a beta-hairpin and a carboxy-terminal loop. On binding DNA, the inducible regions undergo marked conformational changes, becoming part of extended and distorted alpha-helices, which encase the DNA. SarA recognizes an AT-rich site in which the DNA is highly overwound and adopts a D-DNA-like conformation by indirect readout. These structures thus provide insight into SarA-mediated transcription regulation.
About this Structure
1FZP is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.
Reference
Crystal structures of SarA, a pleiotropic regulator of virulence genes in S. aureus., Schumacher MA, Hurlburt BK, Brennan RG, Nature. 2001 Jan 11;409(6817):215-9. PMID:11196648
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