1g1i
From Proteopedia
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|PDB= 1g1i |SIZE=350|CAPTION= <scene name='initialview01'>1g1i</scene>, resolution 2.00Å | |PDB= 1g1i |SIZE=350|CAPTION= <scene name='initialview01'>1g1i</scene>, resolution 2.00Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1g1j|1G1J]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g1i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g1i OCA], [http://www.ebi.ac.uk/pdbsum/1g1i PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1g1i RCSB]</span> | ||
}} | }} | ||
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[[Category: Nodelman, I M.]] | [[Category: Nodelman, I M.]] | ||
[[Category: Schutt, C E.]] | [[Category: Schutt, C E.]] | ||
| - | [[Category: CA]] | ||
[[Category: homo-tetramer]] | [[Category: homo-tetramer]] | ||
[[Category: metal binding site]] | [[Category: metal binding site]] | ||
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[[Category: parallel coiled-coil]] | [[Category: parallel coiled-coil]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:34:44 2008'' |
Revision as of 17:34, 30 March 2008
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| , resolution 2.00Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Related: | 1G1J
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF THE OLIGOMERIZATION DOMAIN FROM ROTAVIRUS NSP4
Overview
During the maturation of rotaviral particles, non-structural protein 4 (NSP4) plays a critical role in the translocation of the immature capsid into the lumen of the endoplasmic reticulum. Full-length NSP4 and a 22 amino acid peptide (NSP4(114-135)) derived from this protein have been shown to induce diarrhea in young mice in an age-dependent manner, and may therefore be the agent responsible for rotavirally-induced symptoms. We have determined the crystal structure of the oligomerization domain of NSP4 which spans residues 95 to 137 (NSP4(95-137)). NSP4(95-137) self-associates into a parallel, tetrameric coiled-coil, with the hydrophobic core interrupted by three polar layers occupying a and d-heptad positions. Side-chains from two consecutive polar layers, consisting of four Gln123 and two of the four Glu120 residues, coordinate a divalent cation. Two independent structures built from MAD-phased data indicated the presence of a strontium and calcium ion bound at this site, respectively. This metal-binding site appears to play an important role in stabilizing the homo-tetramer, which has implications for the engagement of NSP4 as an enterotoxin.
About this Structure
1G1I is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
Crystal structure of the oligomerization domain of NSP4 from rotavirus reveals a core metal-binding site., Bowman GD, Nodelman IM, Levy O, Lin SL, Tian P, Zamb TJ, Udem SA, Venkataraghavan B, Schutt CE, J Mol Biol. 2000 Dec 15;304(5):861-71. PMID:11124032
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