Sandbox Wabash3

(Difference between revisions)

Revision as of 23:16, 14 February 2016

↑ Hanson, R. M., Prilusky, J., Renjian, Z., Nakane, T. and Sussman, J. L. (2013), JSmol and the Next-Generation Web-Based Representation of 3D Molecular Structure as Applied to Proteopedia. Isr. J. Chem., 53:207-216. doi:http://dx.doi.org/10.1002/ijch.201300024
↑ Herraez A. Biomolecules in the computer: Jmol to the rescue. Biochem Mol Biol Educ. 2006 Jul;34(4):255-61. doi: 10.1002/bmb.2006.494034042644. PMID:21638687 doi:10.1002/bmb.2006.494034042644

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 ==Mechanism of Trypsin==
 <StructureSection load='1stp' size='340' side='right' caption='Caption for this structure' scene=''>
-Trypsin is serine protease which catalyzes the hydrolysis of peptide bonds via an acylation reaction and a deacylation reaction. The specificity of substrates is determined by the structure of its active site, which contains an aspartic acid (Asp189 '''Sandbox Wabash3'''. Click above on '''edit this page''' to modify. Be careful with the &lt; and &gt; signs.
+Trypsin is serine protease which catalyzes the hydrolysis of peptide bonds of a substrate via an acylation reaction and a deacylation reaction. In the first (acylation) reaction, the nucleophilic serine attacks the substrate scissile bond, forming a tetrahedral intermediate and then a covalent acyl-enzyme with the release of the C-terminal fragment. In the second (deacylation) reaction, a water molecule attacks the acyl-enzyme, leading to a second tetrahedral intermediate followed by release of the N-terminal fragment. The specificity of substrates is determined by the structure of its active site, which contains Ser-195, His-57, and Asp-189. (Asp189 '''Sandbox Wabash3'''. Click above on '''edit this page''' to modify. Be careful with the &lt; and &gt; signs.
 You may include any references to papers as in: the use of JSmol in Proteopedia <ref>DOI 10.1002/ijch.201300024</ref> or to the article describing Jmol <ref>PMID:21638687</ref> to the rescue.