This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
Sandbox Wabash5
From Proteopedia
(Difference between revisions)
(→The Mechanism of Trypsin) |
|||
| Line 2: | Line 2: | ||
<StructureSection load='2agg' size='340' side='right' caption='Caption for this structure' scene=''> | <StructureSection load='2agg' size='340' side='right' caption='Caption for this structure' scene=''> | ||
| - | Trypsin is a serine protease that contains serine, histidine, and aspartic acid residues. Trypsin catalyzes peptide bond hydrolysis through acid catalysis, base catalysis, and covalent catalysis. In the first step serine acts as a nucleophile to attack the substrate peptide bond, which forms a tetrahedral intermediate via covalent catalysis. Then a acyl-enzyme intermediate is formed and the C-terminal fragment is released. A water molecule attacks the acyl enzyme leading to the creation of a second tetrahedral intermediate. This is followed by the release of the N-terminal fragment, which results in the creation of the active enzyme. | + | Trypsin is a serine protease that contains serine, histidine<scene name='72/725334/Beidou_test_his/3'>HIS</scene>, and aspartic acid residues. Trypsin catalyzes peptide bond hydrolysis through acid catalysis, base catalysis, and covalent catalysis. In the first step serine acts as a nucleophile to attack the substrate peptide bond, which forms a tetrahedral intermediate via covalent catalysis. Then a acyl-enzyme intermediate is formed and the C-terminal fragment is released. A water molecule attacks the acyl enzyme leading to the creation of a second tetrahedral intermediate. This is followed by the release of the N-terminal fragment, which results in the creation of the active enzyme. |
BY: MICHAEL GREEN AND AARON BECKER | BY: MICHAEL GREEN AND AARON BECKER | ||
Revision as of 05:37, 15 February 2016
The Mechanism of Trypsin
| |||||||||||
