1g4c
From Proteopedia
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|PDB= 1g4c |SIZE=350|CAPTION= <scene name='initialview01'>1g4c</scene>, resolution 1.65Å | |PDB= 1g4c |SIZE=350|CAPTION= <scene name='initialview01'>1g4c</scene>, resolution 1.65Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/2-amino-4-hydroxy-6-hydroxymethyldihydropteridine_diphosphokinase 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.6.3 2.7.6.3] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/2-amino-4-hydroxy-6-hydroxymethyldihydropteridine_diphosphokinase 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.6.3 2.7.6.3] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1hka|1HKA]], [[1eqm|1EQM]], [[1eq0|1EQ0]], [[1eqo|1EQO]], [[1ex8|1EX8]], [[1cbk|1CBK]], [[1dy3|1DY3]], [[1f9h|1F9H]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g4c OCA], [http://www.ebi.ac.uk/pdbsum/1g4c PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1g4c RCSB]</span> | ||
}} | }} | ||
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[[Category: Blaszczyk, J.]] | [[Category: Blaszczyk, J.]] | ||
[[Category: Ji, X.]] | [[Category: Ji, X.]] | ||
| - | + | [[Category: 6-hydroxymethyl-7,8-dihydropterin]] | |
| - | + | ||
| - | [[Category: 6-hydroxymethyl-7 | + | |
| - | + | ||
[[Category: antimicrobial agent]] | [[Category: antimicrobial agent]] | ||
[[Category: drug design]] | [[Category: drug design]] | ||
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[[Category: x-ray crystallography]] | [[Category: x-ray crystallography]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:36:21 2008'' |
Revision as of 17:36, 30 March 2008
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| , resolution 1.65Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Activity: | 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase, with EC number 2.7.6.3 | ||||||
| Related: | 1HKA, 1EQM, 1EQ0, 1EQO, 1EX8, 1CBK, 1DY3, 1F9H
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF A COMPLEX OF HPPK(R92A) FROM E.COLI WITH MG2+ AT 1.65 ANGSTROM RESOLUTION
Overview
6-Hydroxymethyl-7,8-dihydropterin pyrophosphokinase (HPPK) catalyzes the pyrophosphoryl transfer from ATP to 6-hydroxymethyl-7,8-dihydropterin (HP), the first reaction in the folate biosynthetic pathway. Arginine residues 82 and 92, strictly conserved in 35 HPPK sequences, play dynamic roles in the catalytic cycle of the enzyme. At 0.89-A resolution, two distinct conformations are observed for each of the two residues in the crystal structure of the wild-type HPPK in complex with two HP variants, two Mg(2+) ions, and an ATP analogue. Structural information suggests that R92 first binds to the alpha-phosphate group of ATP and then shifts to interact with the beta-phosphate as R82, which initially does not bind to ATP, moves in and binds to alpha-phosphate when the pyrophosphoryl transfer is about to occur. The dynamic roles of R82 and R92 are further elucidated by five more crystal structures of two mutant proteins, R82A and R92A, with and without bound ligands. Two oxidized forms of HP are observed with an occupancy ratio of 0.50:0.50 in the 0.89-A structure. The oxidation of HP has significant impact on its binding to the protein as well as the conformation of nearby residue W89.
About this Structure
1G4C is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Dynamic roles of arginine residues 82 and 92 of Escherichia coli 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase: crystallographic studies., Blaszczyk J, Li Y, Shi G, Yan H, Ji X, Biochemistry. 2003 Feb 18;42(6):1573-80. PMID:12578370
Page seeded by OCA on Sun Mar 30 20:36:21 2008
Categories: 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase | Escherichia coli | Single protein | Blaszczyk, J. | Ji, X. | 6-hydroxymethyl-7,8-dihydropterin | Antimicrobial agent | Drug design | Folate | Hppk | Pterin | Pyrophosphokinase | Pyrophosphoryl transfer | X-ray crystallography
