5hy5
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal Structure of a Tryptophan 6-halogenase (SttH) from Streptomyces toxytricini== | |
| + | <StructureSection load='5hy5' size='340' side='right' caption='[[5hy5]], [[Resolution|resolution]] 2.68Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5hy5]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HY5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HY5 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hy5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hy5 OCA], [http://pdbe.org/5hy5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hy5 RCSB], [http://www.ebi.ac.uk/pdbsum/5hy5 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Flavin-dependent halogenase enzymes are potentially useful biocatalysts for the regioselectively halogenation of aromatic compounds. Haloaromatic compounds can be utilised in the synthesis and biosynthesis of pharmaceuticals and other valuable products. Here we report the first X-ray crystal structure of a tryptophan 6-halogenase (SttH), which enabled key residues that contribute to the regioselectivity in tryptophan halogenases to be identified. Structure-guided mutagenesis resulted in a triple mutant (L460F/P461E/P462T) that exhibited a complete switch in regioselectivity; with the substrate 3-indolepropionate 75% 5-chlorination was observed with the mutant in comparison with 90% 6-chlorination for the wild-type SttH. This is the first clear example of how regiocomplementary halogenase enzymes can be created from a single parent enzyme. The biocatalytic repertoire of SttH was also expanded to include a range of indolic and non-indolic substrates. | ||
| - | + | A Structure-Guided Switch in the Regioselectivity of a Tryptophan Halogenase Enzyme.,Shepherd SA, Menon BR, Fisk H, Struck AW, Levy C, Leys D, Micklefield J Chembiochem. 2016 Feb 3. doi: 10.1002/cbic.201600051. PMID:26840773<ref>PMID:26840773</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| + | <div class="pdbe-citations 5hy5" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Levy, C]] | [[Category: Levy, C]] | ||
| + | [[Category: Oxidoreductase]] | ||
| + | [[Category: Tryptophan halogenase]] | ||
Revision as of 02:42, 21 February 2016
Crystal Structure of a Tryptophan 6-halogenase (SttH) from Streptomyces toxytricini
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